Evidence map›Paper›PMID 29540573›Full record

ArticleJournal of lipid research2018

A novel assay for the introduction of the vinyl ether double bond into plasmalogens using pyrene-labeled substrates.

Ernst R Werner, Markus A Keller, Sabrina Sailer, Daniele Seppi, Georg Golderer, Gabriele Werner-Felmayer, Raphael A Zoeller, Katrin Watschinger

Open access · hybridAbstract read
In one paragraph

Article in Journal of lipid research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
  2. Observational
  3. Article
  4. Bifunctional Probes Reveal the Rules of Intracellular Ether Lipid Transport.Angewandte Chemie (International ed. in English) · 2025
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Frontiers in microbiology · 2024
    Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. TheProceedings of the National Academy of Sciences of the United States of America · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Ernst R WernerDivision of Biological Chemistry, Biocenter Medical University of Innsbruck, Innsbruck, Austria; ernst.r.werner@i-med.ac.at katrin.watschinger@i-med.ac.at.ORCID 0000-0003-1948-3391
Markus A KellerDivision of Human Genetics, Medical University of Innsbruck, Innsbruck, Austria; and.ORCID 0000-0002-8654-9920
Sabrina SailerDivision of Biological Chemistry, Biocenter Medical University of Innsbruck, Innsbruck, Austria.
Daniele SeppiDivision of Biological Chemistry, Biocenter Medical University of Innsbruck, Innsbruck, Austria.
Georg GoldererDivision of Biological Chemistry, Biocenter Medical University of Innsbruck, Innsbruck, Austria.
Gabriele Werner-FelmayerDivision of Biological Chemistry, Biocenter Medical University of Innsbruck, Innsbruck, Austria.
Raphael A ZoellerDepartment of Physiology and Biophysics, Boston University School of Medicine, Boston MA.ORCID 0000-0002-6560-0993
Katrin WatschingerDivision of Biological Chemistry, Biocenter Medical University of Innsbruck, Innsbruck, Austria; ernst.r.werner@i-med.ac.at katrin.watschinger@i-med.ac.at.ORCID 0000-0002-1122-8444
Innsbruck Medical University · ATBoston University · US

Funding

Austrian Science Fund FWF P 29551
6 · The paper itself

Abstract

Plasmanylethanolamine desaturase (PEDS) (EC 1.14.99.19) introduces the 1-prime double bond into plasmalogens, one of the most abundant phospholipids in the human body. This labile membrane enzyme has not been purified and its coding sequence is unknown. Previous assays for this enzyme used radiolabeled substrates followed by multistep processing. We describe here a straight-forward method for the quantification of PEDS in enzyme incubation mixtures using pyrene-labeled substrates and reversed-phase HPLC with fluorescence detection. After stopping the reaction with hydrochloric acid in acetonitrile, the mixture was directly injected into the HPLC system without the need of lipid extraction. The substrate, 1-

Indexed as

Chromatography, High Pressure LiquidSpectrometry, FluorescenceAnimalsCells, CulturedMiceMolecular StructureOxidoreductasesPlasmalogensPyrenesSubstrate SpecificityVinyl CompoundsOxidoreductasesPlasmalogensplasmanylethanolamine desaturasepyrenePyrenesVinyl Compoundsvinyl etherether lipidlyso-phosphatidyl ethanolaminephosphatidyl ethanolamineplasmanylethanolamine desaturase

Identifiers

PMID29540573
PMCPMC5928432
OpenAlexW2794430667

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.