ArticleBioscience reports2018
Morphofunctional properties of a differentiated Caco2/HT-29 co-culture as an
Article in Bioscience reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Uptake and Transport of Monotropein and Monotropein Esters in a Caco-2/HT29-MTX-E12 Co-Culture Model.Molecular nutrition & food research · 2026Article
- AdipoRon, an Adiponectin Receptor Agonist, Promotes Intestinal Epithelial Differentiation in Caco-2 Colorectal Adenocarcinoma Cells.Biomedicines · 2026Article
- A Defined Synbiotic Produces Immunomodulatory Metabolites, Engages Gut-Immune Pathways Relevant to Inflammaging, and Supports Healthy Aging in a Nematode Model.International journal of molecular sciences · 2026Article
- In Vitro Testing of Artificial Sweeteners with Apple and Chokeberry Fruit Juice Extracts for Intestinal Permeability and Glucose Uptake.ACS omega · 2026Article
- Co-Extraction of Policosanols and Phytosterols fromMolecules (Basel, Switzerland) · 2026Article
- Characterization of the role of putativeJournal of bacteriology · 2026Article
- A synbiotic medical food improves gut barrier function, reduces immune responses, and inhibits osteoclast activity in models of postmenopausal bone loss aligned with clinical outcomes.Journal of functional foods · 2025Article
- Small Intestine on a Chip Demonstrates Physiologic Mucus Secretion in the Presence of Lacticaseibacillus rhamnosus Biofilm.Biotechnology and bioengineering · 2025Article
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- Ex Vivo, In Vitro and In Vivo Bone Health Properties of Grana Padano Cheese.Foods (Basel, Switzerland) · 2025Article
- Combined BPA and DIBP Exposure Induced Intestinal Mucosal Barrier Impairment Through the Notch Pathway and Gut Microbiota Dysbiosis in Mice.Foods (Basel, Switzerland) · 2025Article
- Surface layer proteins fromTissue barriers · 2024Article
- Detailed survey of aniScience · 2024Article
- HumanTissue barriers · 2024Review
- What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells?BMC microbiology · 2024Article
- Unveiling the Probiotic Potential ofMicroorganisms · 2024Article
- Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology.Frontiers in molecular biosciences · 2024Article
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Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An intestinal 70/30 Caco2/HT-29 co-culture was set up starting from the parental populations of differentiated cells to mimic the human intestinal epithelium. Co-culture was harvested at confluence 0 (T0) and at 3, 6, 10, and 14 days post confluence after plating (T3, T6, T10, and T14, respectively) for morphological and functional analysis. Transmission electron microscopy revealed different features from T0 to T14: microvilli and a complete junctional apparatus from T6, mucus granules from T3, as also confirmed by PAS/Alcian Blue staining. The specific activity of alkaline phosphatase (ALP), aminopeptidase N (APN), and dipeptidyl peptidase IV (DPPIV) progressively increased after T0, indicating the acquirement of a differentiated and digestive phenotype. Transepithelial electrical resistance (TEER), indicative of the barrier properties of the monolayer, increased from T0 up to T6 reaching values very similar to the human small intestine. The apparent permeability coefficient for Lucifer Yellow (LY), along with morphological analysis, reveals a good status of the tight junctions. At T14, HT-29 cells reduced to 18.4% and formed domes, indicative of transepithelial transport of nutrients. This Caco2/HT-29 co-culture could be considered a versatile and suitable
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