ArticleCell reports2018
Two-Photon Bidirectional Control and Imaging of Neuronal Excitability with High Spatial Resolution In Vivo.
Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 176 citations in OpenAlex.
- Reusable modular architecture enables flexible cognitive operations in the mouse brain and artificial recurrent networks.Nature neuroscience · 2026Article
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Cerebellar activity is triggered by reach endpoint during learning of a complex locomotor task.Nature communications · 2026Article
- Active pixel power control for crosstalk-free all-optical neural interrogation.Nature communications · 2026Article
- Technical development of two-photon optogenetic stimulation and its potential application to brain-machine interfaces.Neurophotonics · 2026Review
- Desensitization of opsin responses during all-optical interrogation depends on imaging parameters.Neurophotonics · 2026Article
- Red-shifted excitation enhances the sensitivity of red genetically encoded CaJournal of biomedical optics · 2025Article
- High-throughput synaptic connectivity mapping using in vivo two-photon holographic optogenetics and compressive sensing.Nature neuroscience · 2025Article
- Reusable modular architecture enables flexible cognitive operations.Research square · 2025Article
- Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Blue-shifted ancyromonad channelrhodopsins for multiplex optogenetics.bioRxiv : the preprint server for biology · 2025Article
- Theoretical analysis of low-power deep synergistic sono-optogenetic excitation of neurons by co-expressing light-sensitive and mechano-sensitive ion-channels.Communications biology · 2025Article
- Distinct Inhibitory Neurons Differently Shape Neuronal Codes for Sound Intensity in the Auditory Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Kilohertz electrical stimulation evokes robust cellular responses like conventional frequencies but distinct population dynamics.Communications biology · 2025Article
- Implantable silicon neural probes with nanophotonic phased arrays for single-lobe beam steering.Communications engineering · 2024Article
- "Distinct inhibitory neurons differently shape neuronal codes for sound intensity in the auditory cortex".bioRxiv : the preprint server for biology · 2024Article
- Closed-loop experiments and brain machine interfaces with multiphoton microscopy.Neurophotonics · 2024Review
- Kalium channelrhodopsins effectively inhibit neurons.Nature communications · 2024Article
- Multicolor two-photon light-patterning microscope exploiting the spatio-temporal properties of a fiber bundle.Biomedical optics express · 2024Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
Abstract
Sensory information is encoded within the brain in distributed spatiotemporal patterns of neuronal activity. Understanding how these patterns influence behavior requires a method to measure and to bidirectionally perturb with high spatial resolution the activity of the multiple neuronal cell types engaged in sensory processing. Here, we combined two-photon holography to stimulate neurons expressing blue light-sensitive opsins (ChR2 and GtACR2) with two-photon imaging of the red-shifted indicator jRCaMP1a in the mouse neocortex in vivo. We demonstrate efficient control of neural excitability across cell types and layers with holographic stimulation and improved spatial resolution by opsin somatic targeting. Moreover, we performed simultaneous two-photon imaging of jRCaMP1a and bidirectional two-photon manipulation of cellular activity with negligible effect of the imaging beam on opsin excitation. This all-optical approach represents a powerful tool to causally dissect how activity patterns in specified ensembles of neurons determine brain function and animal behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.