ArticleASAIO journal (American Society for Artificial Internal Organs : 1992)
Bioengineering Cardiac Tissue Constructs With Adult Rat Cardiomyocytes.
Article in ASAIO journal (American Society for Artificial Internal Organs : 1992). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Insights into the molecular mechanism of pulmonary vein stenosis in pediatric patients with congenital heart disease.JTCVS open · 2025Article
- Application of human cardiac organoids in cardiovascular disease research.Frontiers in cell and developmental biology · 2025Review
- Development of Novel 3D Spheroids for Discrete Subaortic Stenosis.Cardiovascular engineering and technology · 2024Article
- An Integrated Optogenetic and Bioelectronic Platform for Regulating Cardiomyocyte Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- An Integrated Optogenetic and Bioelectronic Platform for Regulating Cardiomyocyte Function.bioRxiv : the preprint server for biology · 2023Article
- Research Progress of Three-Dimensional Bioprinting Artificial Cardiac Tissue.Tissue engineering and regenerative medicine · 2023Review
- Recent advances in biological pumps as a building block for bioartificial hearts.Frontiers in bioengineering and biotechnology · 2023Review
- Current State of the Art in Ventricle Tissue Engineering.Frontiers in cardiovascular medicine · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Bioengineering cardiac tissue constructs with adult cardiomyocytes may help treat adult heart defects and injury. In this study, we fabricated cardiac tissue constructs by seeding adult rat cardiomyocytes on a fibrin gel matrix and analyzed the electromechanical properties of the formed cardiac tissue constructs. Adult rat cardiomyocytes were isolated with a collagenase type II buffer using an optimized Langendorff perfusion system. Cardiac tissue constructs were fabricated using either indirect plating with cardiomyocytes that were cultured for 1 week and dedifferentiated or with freshly isolated cardiomyocytes. The current protocol generated (3.1 ± 0.5) × 10 (n = 5 hearts) fresh cardiomyocytes from a single heart. Tissue constructs obtained by both types of plating contracted up to 30 days, and electrogram (ECG) signals and contractile twitch forces were detected. The constructs bioengineered by indirect plating of dedifferentiated cardiomyocytes produced an ECG R wave amplitude of 15.1 ± 5.2 µV (n = 7 constructs), a twitch force of 70-110 µN, and a spontaneous contraction rate of about 390 bpm. The constructs bioengineered by direct plating of fresh cardiomyocytes generated an ECG R wave amplitude of 6.3 ± 2.5 µV (n = 8 constructs), a twitch force of 40-60 µN, and a spontaneous contraction rate of about 230 bpm. This study successfully bioengineered three-dimensional cardiac tissue constructs using primary adult cardiomyocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.