ArticleJournal of the American Society for Mass Spectrometry2018
Single-Cell Mass Spectrometry Reveals Changes in Lipid and Metabolite Expression in RAW 264.7 Cells upon Lipopolysaccharide Stimulation.
Article in Journal of the American Society for Mass Spectrometry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Review
- Single-cell lipidomics: performance evaluation across four liquid chromatography mass spectrometry (LC-MS) systems.The Analyst · 2025Article
- High-Throughput Fluorescence-Guided Sequential Single-Cell MALDI-ICC Mass Spectrometry.Analytical chemistry · 2025Article
- Single-cell mass spectrometry imaging of lipids in HeLa cells via tapping-mode scanning probe electrospray ionization.Communications chemistry · 2025Article
- Single-Cell Untargeted Lipidomics Using Liquid Chromatography and Data-Dependent Acquisition after Live Cell Selection.Analytical chemistry · 2024Article
- Advances in multimodal mass spectrometry for single-cell analysis and imaging enhancement.FEBS letters · 2024Review
- Mass Spectrometry-based Single-Cell Lipidomics: Advancements, Challenges, and the Path Forward.Trends in analytical chemistry : TRAC · 2023Article
- Metabolomics: A useful tool for ischemic stroke research.Journal of pharmaceutical analysis · 2023Review
- Analytical Techniques for Single-Cell Biochemical Assays of Lipids.Annual review of biomedical engineering · 2023Review
- FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins in cells of different lineages.Journal of lipid research · 2023Article
- Nanocapillary sampling coupled to liquid chromatography mass spectrometry delivers single cell drug measurement and lipid fingerprints.The Analyst · 2023Article
- Mass spectrometry imaging to explore molecular heterogeneity in cell culture.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Single-Cell Multiomics Analysis for Drug Discovery.Metabolites · 2021Review
- Metabolomic profiling of single enlarged lysosomes.Nature methods · 2021Article
- Single cell metabolomics using mass spectrometry: Techniques and data analysis.Analytica chimica acta · 2021Review
- Effect of MALDI matrices on lipid analyses of biological tissues using MALDI-2 postionization mass spectrometry.Journal of mass spectrometry : JMS · 2020Article
- Dynamic Range Expansion by Gas-Phase Ion Fractionation and Enrichment for Imaging Mass Spectrometry.Analytical chemistry · 2020Article
- Laser cleavable probes forChemical science · 2019Article
- Article
- Exploring the Fundamental Structures of Life: Non-Targeted, Chemical Analysis of Single Cells and Subcellular Structures.Angewandte Chemie (International ed. in English) · 2019Review
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Authors and funding
5 authors.
Funding
Abstract
It has been widely recognized that individual cells that exist within a large population of cells, even if they are genetically identical, can have divergent molecular makeups resulting from a variety of factors, including local environmental factors and stochastic processes within each cell. Presently, numerous approaches have been described that permit the resolution of these single-cell expression differences for RNA and protein; however, relatively few techniques exist for the study of lipids and metabolites in this manner. This study presents a methodology for the analysis of metabolite and lipid expression at the level of a single cell through the use of imaging mass spectrometry on a high-performance Fourier transform ion cyclotron resonance mass spectrometer. This report provides a detailed description of the overall experimental approach, including sample preparation as well as the data acquisition and analysis strategy for single cells. Applying this approach to the study of cultured RAW264.7 cells, we demonstrate that this method can be used to study the variation in molecular expression with cell populations and is sensitive to alterations in that expression that occurs upon lipopolysaccharide stimulation. Graphical Abstract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.