ArticleAnalytical and bioanalytical chemistry2018
Insert-based microfluidics for 3D cell culture with analysis.
Article in Analytical and bioanalytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- A quantitative meta-analysis comparing cell models in perfused organ on a chip with static cell cultures.Scientific reports · 2023Pooled it
- Investigation of oxidized low-density lipoprotein (oxLDL)-induced inflammation in atherosclerosis using a paper-based immune-vascular model.Analytical and bioanalytical chemistry · 2026Article
- 3D-Printed Microfluidic-Based Cell Culture System With Analysis to Investigate Macrophage Activation.Electrophoresis · 2025Article
- Evaluation of fused deposition modeling (FDM)-printed devices for microfluidic-based cell culture studies.Analytical and bioanalytical chemistry · 2025Article
- 3D Hepatocyte Model with Composite Nanofibers That Reproduced Human In Vivo Drug Clearance Profiles.ACS pharmacology & translational science · 2025Article
- Micro/Nanorobotics in In Vitro Fertilization: A Paradigm Shift in Assisted Reproductive Technologies.Micromachines · 2024Review
- A Microfluidic Platform for the Time-Resolved Interrogation of Polarized Retinal Pigment Epithelial Cells.Translational vision science & technology · 2023Article
- Microenvironmental Analysis and Control for Local Cells under Confluent Conditions via a Capillary-Based Microfluidic Device.Analytical chemistry · 2022Article
- 3D printed devices with integrated collagen scaffolds for cell culture studies including transepithelial/transendothelial electrical resistance (TEER) measurements.Analytica chimica acta · 2022Article
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- Basement membrane properties and their recapitulation in organ-on-chip applications.Materials today. Bio · 2022Review
- MatriGridBioengineering (Basel, Switzerland) · 2022Review
- Evaluation and optimization of PolyJet 3D-printed materials for cell culture studies.Analytical and bioanalytical chemistry · 2022Article
- A Hybrid Nanofiber/Paper Cell Culture Platform for Building a 3D Blood-brain Barrier Model.Small methods · 2021Article
- A Review of Biomaterials and Scaffold Fabrication for Organ-on-a-Chip (OOAC) Systems.Bioengineering (Basel, Switzerland) · 2021Review
- Recent innovations in cost-effective polymer and paper hybrid microfluidic devices.Lab on a chip · 2021Review
- Flexible Materials for High-Resolution 3D Printing of Microfluidic Devices with Integrated Droplet Size Regulation.ACS applied materials & interfaces · 2021Article
- Electrospun Microfibers Modulate Intracellular Amino Acids in Liver Cells via Integrin β1.Bioengineering (Basel, Switzerland) · 2021Article
- Making quantitative biomicrofluidics from microbore tubing and 3D-printed adapters.Biomicrofluidics · 2021Article
- Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production.Biology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We present an insert-based approach to fabricate scalable and multiplexable microfluidic devices for 3D cell culture and integration with downstream detection modules. Laser-cut inserts with a layer of electrospun fibers are used as a scaffold for 3D cell culture, with the inserts being easily assembled in a 3D-printed fluidic device for flow-based studies. With this approach, the number and types of cells (on the inserts) in one fluidic device can be customized. Moreover, after an investigation (i.e., stimulation) under flowing conditions, the cell-laden inserts can be removed easily for subsequent studies including imaging and cell lysis. In this paper, we first discuss the fabrication of the device and characterization of the fibrous inserts. Two device designs containing two (channel width = 260 μm) and four (channel width = 180 μm) inserts, respectively, were used for different experiments in this study. Cell adhesion on the inserts with flowing media through the device was tested by culturing endothelial cells. Macrophages were cultured and stimulated under different conditions, the results of which indicate that the fibrous scaffolds under flow conditions result in dramatic effects on the amount and kinetics of TNF-α production (after LPS stimulation). Finally, we show that the cell module can be integrated with a downstream absorbance detection scheme. Overall, this technology represents a new and versatile way to culture cells in a more in vivo fashion for in vitro studies with online detection modules. Graphical abstract This paper describes an insert-based microfluidic device for 3D cell culture that can be easily scaled, multiplexed, and integrated with downstream analytical modules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.