ArticleScientific reports2018
Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- RrMYB2 Regulates Drought Stress via RrJMJ12-Dependent Epigenetic Modification in Rosa rugosa.Plant biotechnology journal · 2026Article
- Quantification of EV-associated miRNA in liquid biopsies for biomarker signature development.Extracellular vesicles and circulating nucleic acids · 2026Review
- Validation of suitable reference microRNAs for qRT-PCR inFrontiers in plant science · 2025Article
- Identification and validation of qRT-PCR reference genes for analyzing grape infection with gray mold.BMC genomics · 2024Article
- Interfamily Grafted HybridsPlants (Basel, Switzerland) · 2024Article
- The grapevine miR827a regulates the synthesis of stilbenes by targeting VqMYB14 and gives rise to susceptibility in plant immunity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Predicting abiotic stress-responsive miRNA in plants based on multi-source features fusion and graph neural network.Plant methods · 2024Article
- Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker.Scientific reports · 2024Article
- A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression.Forestry research · 2024Article
- LncRNA SNHG1 knockdown inhibits hyperglycemia induced ferroptosis via miR-16-5p/ACSL4 axis to alleviate diabetic nephropathy.Journal of diabetes investigation · 2023Article
- Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties.Plants (Basel, Switzerland) · 2023Article
- Advances in understanding cold tolerance in grapevine.Plant physiology · 2023Review
- Small RNAs and Karma methylation in Elaeis guineensis mother palms are linked to high clonal mantling.Plant molecular biology · 2023Article
- Canalization of Phenotypes-When the Transcriptome is Constantly but Weakly Perturbed.Molecular biology and evolution · 2023Article
- Longan extract suppresses food intake through regulation of POMC/AgRP neuronal activities and endoplasmic reticulum stress in hypothalamus of db/db mice.Frontiers in nutrition · 2023Article
- Integrated agronomic, physiological, microstructure, and whole-transcriptome analyses reveal the role of biomass accumulation and quality formation during Se biofortification in alfalfa.Frontiers in plant science · 2023Article
- Systematic identification of reference genes for qRT-PCR ofFrontiers in plant science · 2023Article
- Grapevine Leafroll-Associated Virus 3 Replication in Grapevine Hosts Changes through the Dormancy Stage.Plants (Basel, Switzerland) · 2022Article
- Article
- Validation of Reference Genes in a Population of BlueberryPlants (Basel, Switzerland) · 2022Article
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Authors and funding
9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Grapevine is among the fruit crops with high economic value, and because of the economic losses caused by abiotic stresses, the stress resistance of Vitis vinifera has become an increasingly important research area. Among the mechanisms responding to environmental stresses, the role of miRNA has received much attention recently. qRT-PCR is a powerful method for miRNA quantitation, but the accuracy of the method strongly depends on the appropriate reference genes. To determine the most suitable reference genes for grapevine miRNA qRT-PCR, 15 genes were chosen as candidate reference genes. After eliminating 6 candidate reference genes with unsatisfactory amplification efficiency, the expression stability of the remaining candidate reference genes under salinity, cold and drought was analysed using four algorithms, geNorm, NormFinder, deltaCt and Bestkeeper. The results indicated that U6 snRNA was the most suitable reference gene under salinity and cold stresses; whereas miR168 was the best for drought stress. The best reference gene sets for salinity, cold and drought stresses were miR160e + miR164a, miR160e + miR168 and ACT + UBQ + GAPDH, respectively. The selected reference genes or gene sets were verified using miR319 or miR408 as the target gene.
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