Evidence map›Paper›PMID 29535210›Full record

ArticleJournal of cell science2018

Rapid production of pure recombinant actin isoforms in

Tomoyuki Hatano, Salvatore Alioto, Emanuele Roscioli, Saravanan Palani, Scott T Clarke, Anton Kamnev, Juan Ramon Hernandez-Fernaud, Lavanya Sivashanmugam, Bernardo Chapa-Y-Lazo, Alexandra M E Jones and 6 more

Open access · hybridAbstract read
In one paragraph

Article in Journal of cell science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 41 citations in OpenAlex.

  1. Article
  2. Actin isoform-specific interactions revealed byProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
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  6. Review
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  17. Article
  18. Tools of the trade: studying actin in zebrafish.Histochemistry and cell biology · 2020
    Review
  19. Article
  20. Regulation of actin isoforms in cellular and developmental processes.Seminars in cell & developmental biology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 4 countries.

Tomoyuki HatanoCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK T.Hatano@warwick.ac.uk m.k.balasubramanian@warwick.ac.uk.
Salvatore AliotoDepartment of Biology, Brandeis University, Waltham, MA 02454, USA.
Emanuele RoscioliCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Saravanan PalaniCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Scott T ClarkeCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Anton KamnevCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Juan Ramon Hernandez-FernaudSchool of Life Sciences, University of Warwick, Coventry CV4 7AL, UK.
Lavanya SivashanmugamCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Bernardo Chapa-Y-LazoCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Alexandra M E JonesSchool of Life Sciences, University of Warwick, Coventry CV4 7AL, UK.
Robert C RobinsonInstitute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology, and Research), Singapore 138673, Singapore.
Karuna SampathCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Masanori MishimaCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Andrew D McAinshCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
Bruce L GoodeDepartment of Biology, Brandeis University, Waltham, MA 02454, USA.
Mohan K BalasubramanianCentre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK T.Hatano@warwick.ac.uk m.k.balasubramanian@warwick.ac.uk.ORCID 0000-0002-1292-8602
University of Warwick · GBBrandeis University · USAgency for Science, Technology and Research · SG

Funding

MECHNISMS REGULATING ACTIN ASSEMBLYR01GM063691 · NIGMS · BRANDEIS UNIVERSITY · PI GOODE, BRUCE L · 2002 to 2019
$7.5M
NIGMS NIH HHS R01 GM063691Wellcome Trust
6 · The paper itself

Abstract

Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of the non-muscle actin cytoskeleton. Here, we report a rapid and cost-effective method to purify heterologous actins expressed in the yeast

Indexed as

ActinsAnimalsHumansPichiaProtein IsoformsActinsProtein IsoformsActinActin purificationBiochemistryCytoskeleton

Identifiers

PMID29535210
PMCPMC5976186
OpenAlexW2791424423

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.