ArticleAnalytica chimica acta2018
A competitive lateral flow assay for the detection of tenofovir.
Article in Analytica chimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Treatment of Chronic Venous Ulcers With Heterologous Fibrin Sealant: A Phase I/II Clinical Trial.Frontiers in immunology · 2021Trial
- Interplay of the Mass Transport and Reaction Kinetics for Lateral Flow Immunoassay Integrated on Lab-on-Disc.Sensors (Basel, Switzerland) · 2025Article
- Rapid Enzymatic Assay for Antiretroviral Drug Monitoring Using CRISPR-Cas12a-Enabled Readout.ACS synthetic biology · 2025Article
- REverse transcriptase ACTivity (REACT) assay for point-of-care measurement of established and emerging antiretrovirals for HIV treatment and prevention.Analytical and bioanalytical chemistry · 2024Article
- Gold Nanoparticle Paper Immunoassays for Sensing the Presence ofACS omega · 2023Article
- Can precision antibiotic prescribing help prevent the spread of carbapenem-resistant organisms in the hospital setting?JAC-antimicrobial resistance · 2023Review
- Development of a Monoclonal Antibody and a Serodiagnostic Lateral-Flow Device Specific toJournal of fungi (Basel, Switzerland) · 2022Article
- Point-of-care semi-quantitative test for adherence to tenofovir alafenamide or tenofovir disoproxil fumarate.The Journal of antimicrobial chemotherapy · 2022Article
- HIV pre-exposure prophylaxis adherence test using reverse transcription isothermal amplification inhibition assay.Analytical methods : advancing methods and applications · 2022Article
- Tunable Duplex Semiquantitative Detection of Nucleic Acids with a Visual Lateral Flow Immunoassay Readout.Analytical chemistry · 2022Article
- Pilot evaluation of an enzymatic assay for rapid measurement of antiretroviral drug concentrations.Virology journal · 2021Article
- Rapid, user-friendly, and inexpensive detection of azidothymidine.Analytical and bioanalytical chemistry · 2021Article
- Point-of-care and Near Real-time Testing for Antiretroviral Adherence Monitoring to HIV Treatment and Prevention.Current HIV/AIDS reports · 2020Review
- Perspectives on the utility and interest in a point-of-care urine tenofovir test for adherence to HIV pre-exposure prophylaxis and antiretroviral therapy: an exploratory qualitative assessment among U.S. clients and providers.AIDS research and therapy · 2020Article
- Immunoassay for HIV Drug Metabolites Tenofovir and Tenofovir Diphosphate.ACS infectious diseases · 2020Article
- Enzymatic Assay for Rapid Measurement of Antiretroviral Drug Levels.ACS sensors · 2020Article
- Brief Report: Urine Emtricitabine and Tenofovir Concentrations Provide Markers of Recent Antiretroviral Drug Exposure Among HIV-Negative Men Who Have Sex With Men.Journal of acquired immune deficiency syndromes (1999) · 2019Article
- Designing Paper-Based Immunoassays for Biomedical Applications.Sensors (Basel, Switzerland) · 2019Review
- Development and Validation of an Immunoassay for Tenofovir in Urine as a Real-Time Metric of Antiretroviral Adherence.EClinicalMedicineArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Proper management of an HIV infection requires that a patient be at least 80-95% adherent to a prescribed drug regimen to avoid poor health outcomes and the development of drug-resistant HIV strains. Clinicians generally monitor adherence habits indirectly through patient self-reporting, pill counting, and electronic drug monitoring. While direct measurement of patient samples like urine for monitoring drug levels is possible, it requires specialized equipment and training that is not readily available in resource-limited settings where the need is greatest. In this work we report the development of an antibody that binds to tenofovir (TFV), a key small molecule drug for both the treatment and prevention of HIV, and a competitive lateral flow assay that uses that antibody to monitor urine samples for the presence of the drug. TFV was conjugated to an immunogenic protein and injected into rabbits to raise polyclonal antibodies sensitive to the drug. The antibodies were verified for TFV-sensitivity by immunoprecipitation and HPLC. A gold nanoparticle-based competitive assay was developed to detect the presence of TFV in urine samples with a sensitivity of 1 μg mL
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.