Evidence map›Paper›PMID 29534068›Full record

ArticlePloS one2018

The effect of S100A6 on nuclear translocation of CacyBP/SIP in colon cancer cells.

Shanshan Feng, Qiaozhi Zhou, Bo Yang, Qianqian Li, Aiqin Liu, Yingying Zhao, Changqing Qiu, Jun Ge, Huihong Zhai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Shanshan FengSurgery Laboratory, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China.
Qiaozhi ZhouDepartment of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Bo YangDepartment of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Qianqian LiDepartment of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Aiqin LiuDepartment of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Yingying ZhaoDepartment of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Changqing QiuDepartment of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Jun GeDepartment of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Huihong ZhaiDepartment of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.ORCID 0000-0002-8452-6311
Capital Medical University · CNNingxia Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCalcyclin Binding Protein/(Siah-1 interacting protein) (CacyBP/SIP) acts as an oncogene in colorectal cancer. The nuclear accumulation of CacyBP/SIP has been linked to the proliferation of cancer cells. It has been reported that intracellular Ca2+ induces the nuclear translocation of CacyBP/SIP. However, the molecular mechanism of CacyBP/SIP nuclear translocation has yet to be elucidated. The purpose of this study was to test whether the Ca2+-dependent binding partner S100 protein is involved in CacyBP/SIP nuclear translocation in colon cancer SW480 cells.

methodsThe subcellular localization of endogenous CacyBP/SIP was observed following the stimulation of ionomycin or BAPTA/AM by immunofluorescence staining in SW480 cells. S100A6 small interfering RNAs (siRNA) were transfected into SW480 cells. Immunoprecipitation assays detected whether S100 protein is relevant to the nuclear translocation of CacyBP/SIP in response to changes in [Ca2+]i.

resultsWe observed that endogenous CacyBP/SIP is translocated from the cytosol to the nucleus following the elevation of [Ca2+]i by ionomycin in SW480 cells. Co-immunoprecipitation experiments showed that the interaction between S100A6 and CacyBP/SIP was increased simultaneously with elevated Ca2+. Knockdown of S100A6 abolished the Ca2+ effect on the subcellular translocation of CacyBP/SIP.

conclusionThus, we demonstrated that S100A6 is required for the Ca2+-dependent nuclear translocation of CacyBP/SIP in colon cancer SW480 cells.

Indexed as

Active Transport, Cell NucleusCalciumCalcium-Binding ProteinsCalcium IonophoresCell Line, TumorCell NucleusColonic NeoplasmsGene Expression Regulation, NeoplasticHumansIonomycinRNA InterferenceS100 Calcium Binding Protein A6CACYBP protein, humanCalciumCalcium-Binding ProteinsCalcium IonophoresIonomycinS100 Calcium Binding Protein A6

Identifiers

PMID29534068
PMCPMC5849316
OpenAlexW2791084738

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.