Evidence map›Paper›PMID 29532994›Full record

ArticleJournal of cellular and molecular medicine2018

CiRS-7 targeting miR-7 modulates the progression of non-small cell lung cancer in a manner dependent on NF-κB signalling.

Chongyu Su, Yi Han, Hongtao Zhang, Yunsong Li, Ling Yi, Xiaojue Wang, Shijie Zhou, Daping Yu, Xiaoyun Song, Ning Xiao and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed, 5 pooled it
6.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 5 syntheses or guidelines pooled it, 126 citations in OpenAlex.

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14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Chongyu SuDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Yi HanDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Hongtao ZhangDepartment of Central Laboratory, Beijing Chest Hospital, Capital Medical University and Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
Yunsong LiDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Ling YiDepartment of Central Laboratory, Beijing Chest Hospital, Capital Medical University and Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
Xiaojue WangDepartment of Central Laboratory, Beijing Chest Hospital, Capital Medical University and Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
Shijie ZhouDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Daping YuDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Xiaoyun SongDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Ning XiaoDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Xiaoqing CaoDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.
Zhidong LiuDepartment of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing, China.ORCID 0000-0003-2416-570X
Capital Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The purpose of this study was to figure out the effect of ciRS-7/miR-7/NF-κB axis on the development of non-small cell lung cancer (NSCLC). In response, the expressions of ciRS-7, miR-7 and NF-κB subunit (ie RELA) within NSCLC tissues and cell lines were determined with real-time polymerase chain reaction (RT-PCR) and Western blot. Moreover, the NSCLC cells were transfected with pcDNA3-ciRS-7-ir, pcDNA3-ciRS-7, miR-NC and miR-7 mimic. Furthermore, the targeted relationships between ciRS-7 and miR-7, as well as between miR-7 and RELA, were confirmed by luciferase reporter assay. The proliferation, migration and apoptosis of NSCLC cells were, successively, measured using CCK-8 assay, wound-healing assay and flow cytometry test. Consequently, ciRS-7, miR-7, histopathological grade, lymph node metastasis and histopathological stage could independently predict the prognosis of patients with NSCLC (all P < .05). Moreover, remarkably up-regulated ciRS-7 and RELA expressions, as along with down-regulated miR-7 expressions, were found within NSCLC tissues and cells in comparison with normal ones (P < .05). Besides, overexpressed ciRS-7 and underexpressed miR-7 were correlated with increased proliferation, migration and invasion, yet reduced apoptosis rate of NSCLC cells (P < .05). More than that, ciRS-7 specifically targeted miR-7 to reduce its expressions (P < .05). Ultimately, the NSCLC cells within miR-7 + RELA group were observed with superior proliferative, migratory and invasive capabilities than those within miR-7 group (P < .05), and RELA expression was also significantly modified by both ciRS-7 and miR-7 (P < .05). In conclusion, the ciRS-7/miR-7/NF-kB axis could exert pronounced impacts on the proliferation, migration, invasion and apoptosis of NSCLC cells.

Indexed as

A549 CellsAgedApoptosisCarcinoma, Non-Small-Cell LungCell MovementCell ProliferationDisease-Free SurvivalFemaleGene Expression Regulation, NeoplasticHumansKaplan-Meier EstimateMaleMicroRNAsMiddle AgedNeoplasm InvasivenessNF-kappa Blong non-coding RNA CDR1AS, humanMicroRNAsMIRN7-1 microRNA, humanNF-kappa BRELA protein, humanRNA, Long NoncodingTranscription Factor RelAcell activitycell apoptosiscell invasioncell migrationCiRS-7miR-7NF-kBnon-small cell lung cancer

Identifiers

PMID29532994
PMCPMC5980210
OpenAlexW2792204919

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.