ArticlePlant, cell & environment2018
How does temperature affect splicing events? Isoform switching of splicing factors regulates splicing of LATE ELONGATED HYPOCOTYL (LHY).
Article in Plant, cell & environment, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Divergent molecular pathways govern temperature-dependent wheat stem rust resistance genes.Nature communications · 2025Article
- Abundant clock proteins point to missing molecular regulation in the plant circadian clock.Molecular systems biology · 2025Article
- Untranslated yet indispensable-UTRs act as key regulators in the environmental control of gene expression.Journal of experimental botany · 2024Review
- Inhibition of RNA degradation integrates the metabolic signals induced by osmotic stress into the Arabidopsis circadian system.Journal of experimental botany · 2023Article
- Alternative Splicing in the Regulatory Circuit of Plant Temperature Response.International journal of molecular sciences · 2023Review
- Major transcriptomic differences are induced by warmer temperature conditions experienced during asexual and sexual reproduction inFrontiers in plant science · 2023Article
- Circadian entrainment in Arabidopsis.Plant physiology · 2022Article
- High-quality reference transcriptome construction improves RNA-seq quantification inFrontiers in genetics · 2022Article
- The Evolution of Hemocyanin Genes in Caenogastropoda: Gene Duplications and Intron Accumulation in Highly Diverse Gastropods.Journal of molecular evolution · 2021Article
- The evolution of hemocyanin genes in Tectipleura: a multitude of conserved introns in highly diverse gastropods.BMC ecology and evolution · 2021Article
- PacBio and Illumina RNA Sequencing Identify Alternative Splicing Events in Response to Cold Stress in Two Poplar Species.Frontiers in plant science · 2021Article
- Temperature-Dependent Alternative Splicing of Precursor mRNAs and Its Biological Significance: A Review Focused on Post-Transcriptional Regulation of a Cold Shock Protein Gene in Hibernating Mammals.International journal of molecular sciences · 2020Review
- PIF4 and HOOKLESS1 Impinge on Common Transcriptome and Isoform Regulation in Thermomorphogenesis.Plant communications · 2020Article
- The Plant Circadian Oscillator.Biology · 2019Review
- Thermo-Sensitive Alternative Splicing ofFrontiers in plant science · 2019Article
- How does temperature affect splicing events? Isoform switching of splicing factors regulates splicing of LATE ELONGATED HYPOCOTYL (LHY).Plant, cell & environment · 2018Article
- Global spatial analysis of Arabidopsis natural variants implicates 5'UTR splicing of LATE ELONGATED HYPOCOTYL in responses to temperature.Plant, cell & environment · 2018Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
One of the ways in which plants can respond to temperature is via alternative splicing (AS). Previous work showed that temperature changes affected the splicing of several circadian clock gene transcripts. Here, we investigated the role of RNA-binding splicing factors (SFs) in temperature-sensitive AS of the clock gene LATE ELONGATED HYPOCOTYL (LHY). We characterized, in wild type plants, temperature-associated isoform switching and expression patterns for SF transcripts from a high-resolution temperature and time series RNA-seq experiment. In addition, we employed quantitative RT-PCR of SF mutant plants to explore the role of the SFs in cooling-associated AS of LHY. We show that the splicing and expression of several SFs responds sufficiently, rapidly, and sensitively to temperature changes to contribute to the splicing of the 5'UTR of LHY. Moreover, the choice of splice site in LHY was altered in some SF mutants. The splicing of the 5'UTR region of LHY has characteristics of a molecular thermostat, where the ratio of transcript isoforms is sensitive to temperature changes as modest as 2 °C and is scalable over a wide dynamic range of temperature. Our work provides novel insight into SF-mediated coupling of the perception of temperature to post-transcriptional regulation of the clock.
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