Evidence map›Paper›PMID 29532174›Full record

ArticlePharmaceutical research2018

Blood-to-Retina Transport of Fluorescence-Labeled Verapamil at the Blood-Retinal Barrier.

Yoshiyuki Kubo, Ayumi Nakazawa, Shin-Ichi Akanuma, Ken-Ichi Hosoya

Abstract read
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In one paragraph

Article in Pharmaceutical research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yoshiyuki KuboDepartment of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630, Sugitani, Toyama, 930-0194, Japan.
Ayumi NakazawaDepartment of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630, Sugitani, Toyama, 930-0194, Japan.
Shin-Ichi AkanumaDepartment of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630, Sugitani, Toyama, 930-0194, Japan.
Ken-Ichi HosoyaDepartment of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630, Sugitani, Toyama, 930-0194, Japan. hosoyak@pha.u-toyama.ac.jp.

Funding

Japan Society for the Promotion of Science JP16H05110Japan Society for the Promotion of Science JP17K08409
6 · The paper itself

Abstract

purposeTo investigate the blood-to-retina verapamil transport at the blood-retinal barrier (BRB).

methodsEverFluor FL Verapamil (EFV) was adopted as the fluorescent probe of verapamil, and its transport across the BRB was investigated with common carotid artery infusion in rats. EFV transport at the inner and outer BRB was investigated with TR-iBRB2 cells and RPE-J cells, respectively.

resultsThe signal of EFV was detected in the retinal tissue during the weak signal of cell impermeable compound. In TR-iBRB2 cells, the localization of EFV differed from that of LysoTracker

conclusionsOur findings provide visual evidence to support the significance of carrier-mediated transport in the blood-to-retina verapamil transport at the BRB.

Indexed as

AnimalsBlood-Retinal BarrierCalcium Channel BlockersCell LineFluorescent DyesMaleMicroscopy, ConfocalMicroscopy, FluorescenceModels, AnimalPermeabilityRatsRats, WistarRetinal Pigment EpitheliumVerapamilCalcium Channel BlockersFluorescent DyesVerapamilblood-retinal barrierlysosomal trappingneuroprotectantsorganic cation transportverapamil

Identifiers

PMID29532174

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.