ArticleEcology and evolution2018
Improved protocols to accelerate the assembly of DNA barcode reference libraries for freshwater zooplankton.
Article in Ecology and evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 74 citations in OpenAlex.
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- Cohabitation with aggressive hosts: description of a new microhisterid species in nests of a ponerine ant with ecological notes.Scientific reports · 2023Article
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- The Effects of Water Level Fluctuation on Zooplankton Communities in Shahu Lake Based on DNA Metabarcoding and Morphological Methods.Animals : an open access journal from MDPI · 2022Article
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- A new set of highly efficient primers for COI amplification in rotifers.Mitochondrial DNA. Part B, Resources · 2021Article
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- Using high-resolution melting to identify Calliphoridae (blowflies) species from Brazil.PeerJ · 2020Article
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- Improved protocols to accelerate the assembly of DNA barcode reference libraries for freshwater zooplankton.Ecology and evolution · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently, freshwater zooplankton sampling and identification methodologies have remained virtually unchanged since they were first established in the beginning of the XX century. One major contributing factor to this slow progress is the limited success of modern genetic methodologies, such as DNA barcoding, in several of the main groups. This study demonstrates improved protocols which enable the rapid assessment of most animal taxa inhabiting any freshwater system by combining the use of light traps, careful fixation at low temperatures using ethanol, and zooplankton-specific primers. We DNA-barcoded 2,136 specimens from a diverse array of taxonomic assemblages (rotifers, mollusks, mites, crustaceans, insects, and fishes) from several Canadian and Mexican lakes with an average sequence success rate of 85.3%. In total, 325 Barcode Index Numbers (BINs) were detected with only three BINs (two cladocerans and one copepod) shared between Canada and Mexico, suggesting a much narrower distribution range of freshwater zooplankton than previously thought. This study is the first to broadly explore the metazoan biodiversity of freshwater systems with DNA barcodes to construct a reference library that represents the first step for future programs which aim to monitor ecosystem health, track invasive species, or improve knowledge of the ecology and distribution of freshwater zooplankton.
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