ArticleNature communications2018
Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Development of a Microdroplet-Based Functional Genomic Screening Pipeline by Combination of DNA Nanoflowers and PURExpress Cell-Free Expression.ACS synthetic biology · 2026Article
- Artificial intelligence and automation in enzyme engineering: evolution, advances, and future perspectives.Bioresources and bioprocessing · 2026Review
- Sensitive, high-throughput, metabolic analysis by molecular sensors on the membrane surface of mother yeast cells.Nature communications · 2025Article
- Combing Directed Enzyme Evolution with Metabolic Engineering to Develop Efficient Microbial Cell Factories.Chem & bio engineering · 2025Review
- Massively parallel genetic perturbation suggests the energetic structure of an amyloid-β transition state.Science advances · 2025Article
- Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets.Journal of the American Chemical Society · 2025Article
- FACS-Sortable Triple Emulsion Picoreactors for Screening Reactions in Biphasic Environments.Advanced materials interfaces · 2025Article
- Enhanced stereodivergent evolution of carboxylesterase for efficient kinetic resolution of near-symmetric esters through machine learning.Nature communications · 2024Article
- Droplet Microfluidics for High-Throughput Screening and Directed Evolution of Biomolecules.Micromachines · 2024Review
- Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins.Nature chemistry · 2024Article
- On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale.Sensors (Basel, Switzerland) · 2024Review
- Advances in ligand-specific biosensing for structurally similar molecules.Cell systems · 2023Review
- Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system.World journal of microbiology & biotechnology · 2023Review
- Particle Counting Methods Based on Microfluidic Devices.Micromachines · 2023Review
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Homogeneous, heterogeneous, and enzyme catalysis in microfluidics droplets.Smart molecules : open access · 2023Review
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- The Role of the Residue at Position 2 in the Catalytic Activity of AA9 Lytic Polysaccharide Monooxygenases.International journal of molecular sciences · 2023Article
- Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies.Analytical chemistry · 2023Article
- Strain Development in Microalgal Biotechnology-Random Mutagenesis Techniques.Life (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Directed evolution has long been a key strategy to generate enzymes with desired properties like high selectivity, but experimental barriers and analytical costs of screening enormous mutant libraries have limited such efforts. Here, we describe an ultrahigh-throughput dual-channel microfluidic droplet screening system that can be used to screen up to ~10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.