ArticleParasites & vectors2018
Transcriptome analysis of pig intestinal cell monolayers infected with Cryptosporidium parvum asexual stages.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 27 citations in OpenAlex.
- Exploring the repertoire of rhomboid proteases inFrontiers in cellular and infection microbiology · 2026Article
- Host cell KRT-18 as a non-redox dependent interactor of Cryptosporidium parvum PDI enables parasite infection.Communications biology · 2025Article
- Gene expression profile of HCT-8 cells following single or co-infections with Cryptosporidium parvum and bovine coronavirus.Scientific reports · 2023Article
- Combination of inhibitors for two glycolytic enzymes portrays high synergistic efficacy againstAntimicrobial agents and chemotherapy · 2023Article
- Editorial: Host response to veterinary infectious diseases: role of coding and non-coding RNAs as biomarkers and disease modulators.Frontiers in veterinary science · 2023Article
- An update onJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- IsFood and waterborne parasitology · 2022Article
- Small and intermediate size structural RNAs in the unicellular parasiteMicrobial genomics · 2022Article
- Proteome-wide prediction and analysis of theComputational and structural biotechnology journal · 2022Article
- Transcriptome Analysis and Autophagy Investigation of LoVo Cells Stimulated with Exosomes Derived fromMicroorganisms · 2021Article
- Noncoding RNAs in Apicomplexan Parasites: An Update.Trends in parasitology · 2020Review
- Article
- Analysis of Long Non-Coding RNA inFrontiers in cellular and infection microbiology · 2020Article
- Transcriptomic Study of Porcine Small Intestine Epithelial Cells Reveals Important Genes and Pathways Associated With Susceptibility toFrontiers in genetics · 2020Article
- Cryptosporidium parvum alters glucose transport mechanisms in infected enterocytes.Parasitology research · 2019Article
- Comparative Pathobiology of the Intestinal Protozoan ParasitesPathogens (Basel, Switzerland) · 2019Review
- Primary Cell-Derived Intestinal Models: Recapitulating Physiology.Trends in biotechnology · 2019Review
- The transcriptome of Cryptosporidium oocysts and intracellular stages.Scientific reports · 2019Article
- ApiAP2 Transcription Factors in Apicomplexan Parasites.Pathogens (Basel, Switzerland) · 2019Review
- Mammalian Annotation Database for improved annotation and functional classification of Omics datasets from less well-annotated organisms.Database : the journal of biological databases and curation · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundHuman cryptosporidiosis is caused primarily by two species of apicomplexan protozoa, Cryptosporidium parvum and C. hominis. In cultured cell monolayers, the parasite undergoes two generations of asexual multiplication (merogony). However, the proportion of parasites completing the life-cycle is low and insufficient to sustain continuous propagation. Due to the intracellular location of meronts and later life-cycle stages, oocyst and sporozoites are the only forms of the parasite that can readily be isolated.
resultsResearch on the replicating forms of Cryptosporidium parasites and their interaction with the host cell remains challenging. Based on an RNA-Seq analysis of monolayers of pig epithelial cells infected with C. parvum, here we report on the impact of merogony on the host's gene regulation. Analysis of the transcriptome of infected and uninfected monolayers demonstrates a significant impact of the infection on host cell gene expression. A total of 813 genes were differentially expressed. Functional terms significantly altered in response to infection include phosphoprotein, RNA binding and acetylation. Upregulation of cell cycle pathways indicates an increase in mitosis. Notably absent from differentially enriched functional categories are stress- and apoptosis-related functions. The comparison of the combined host-parasite transcriptome reveals that C. parvum gene expression is less diverse than the host cell transcriptome and is highly enriched for genes encoding ribosomal functions, such as ribosomal proteins.
conclusionsThese results indicate that C. parvum infection significantly changes host biological functions and provide new insight into gene functions driving early C. parvum intracellular development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.