ArticleParasites & vectors2018
Microarray analysis of long non-coding RNA expression profiles uncovers a Toxoplasma-induced negative regulation of host immune signaling.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Non-coding RNAs in parasitic diseases: a bibliometric analysis of knowledge structure, research hotspots, and future directions.Frontiers in cellular and infection microbiology · 2026Pooled it
- Circular RNAs: An Under-Recognized Part in Different Scenarios of Host-Parasite Interrelationships.Pathogens (Basel, Switzerland) · 2026Review
- Unveiling the Hidden Regulators: The Impact of lncRNAs on Zoonoses.International journal of molecular sciences · 2024Review
- Expression profiles of host miRNAs and circRNAs and ceRNA network during Toxoplasma gondii lytic cycle.Parasitology research · 2024Article
- Dynamic RNA profiles in the small intestinal epithelia of cats after Toxoplasma gondii infection.Infectious diseases of poverty · 2023Article
- A Systematic Review of Apicomplexa Looking into Epigenetic Pathways and the Opportunity for Novel Therapies.Pathogens (Basel, Switzerland) · 2023Review
- Article
- RNA sequencing reveals dynamic expression of lncRNAs and mRNAs in caprine endometrial epithelial cells induced by Neospora caninum infection.Parasites & vectors · 2022Article
- Temporal transcriptomic changes in long non-coding RNAs and messenger RNAs involved in the host immune and metabolic response during Toxoplasma gondii lytic cycle.Parasites & vectors · 2022Article
- Identification and characterization of novel infection associated transcripts in macrophages.RNA biology · 2021Article
- Decoding LncRNAs.Cancers · 2021Review
- Transcriptomics analysis of Toxoplasma gondii-infected mouse macrophages reveals coding and noncoding signatures in the presence and absence of MyD88.BMC genomics · 2021Article
- Article
- Functional Intricacy and Symmetry of Long Non-Coding RNAs in Parasitic Infections.Frontiers in cellular and infection microbiology · 2021Review
- Transcriptome Analysis Revealed Potential Mechanisms of Resistance toFrontiers in veterinary science · 2021Article
- miRNAs and lncRNAs inInternational journal of molecular sciences · 2020Review
- Expression of Long Non-Coding RNAs by Human Retinal Müller Glial Cells Infected with Clonal and Exotic VirulentNon-coding RNA · 2019Article
- Long Non-Coding RNAs in the Regulation of Gene Expression: Physiology and Disease.Non-coding RNA · 2019Review
- Impact ofFrontiers in cellular and infection microbiology · 2019Review
- Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundToxoplasma gondii is an obligate intracellular protozoan parasite that can infect mammalian cells and thereby regulate host gene expression. The long non-coding RNAs (lncRNAs) have been demonstrated to be an important class of RNA molecules that regulate many biological processes, including host-pathogen interactions. However, the role of host lncRNAs in the response to T. gondii infection remains largely unknown.
methodsWe applied a microarray approach to determine the differential expression profiles of both lncRNAs and mRNAs in the human foreskin fibroblast (HFF) cells after T. gondii infection. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to reveal the potential functions of T. gondii-induced genes. Based on the co-expression networks of lncRNAs and immune-related genes, the role of NONSHAT022487 on the regulation of UNC93B1 related immune signaling was investigated by the knockdown and over-expression of lncRNA in human macrophage derived from the PMA-induced promonocytic cell line THP-1.
resultsOur data showed that 996 lncRNAs and 109 mRNAs in HFF cells were significantly and differentially expressed following T. gondii infection (fold change ≥ 5, P < 0.05). The results from the GO and KEGG pathway analyses indicated that the mRNAs with differential expression were mainly involved in the host immune response. Remarkably, we identified a novel lncRNA, NONSHAT022487, which suppresses the expression of the immune-related molecule UNC93B1. After T. gondii infection, NONSHAT022487 impaired the secretion of the cytokines IL-12, TNF-α, IL-1β and IFN-γ by downregulating UNC93B1 expression in human macrophage cells.
conclusionsOur study identified infection-induced lncRNA expression as a novel mechanism by which the Toxoplasma parasite regulates host immune signaling, which advances our understanding of the interaction of T. gondii parasites and host cells.
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