Evidence map›Paper›PMID 29530027›Full record

ArticleBMC complementary and alternative medicine2018

Inhibition of lipopolysaccharide (LPS)-induced neuroinflammatory response by polysaccharide fractions of Khaya grandifoliola (C.D.C.) stem bark, Cryptolepis sanguinolenta (Lindl.) Schltr and Cymbopogon citratus Stapf leaves in raw 264.7 macrophages and U87 glioblastoma cells.

Francine Kengne Mediesse, Thaddée Boudjeko, Anantharaju Hasitha, Matharasala Gangadhar, Wilfred Fon Mbacham, Perumal Yogeeswari

Open access · goldAbstract read
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In one paragraph

Article in BMC complementary and alternative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Francine Kengne MediesseLaboratory of Phytoprotection and Valorisation of Plant Resources, The Biotechnology Centre, University of Yaounde I, P.O. Box 3851, Messa-Yaounde, Yaounde, Cameroon.
Thaddée BoudjekoLaboratory of Phytoprotection and Valorisation of Plant Resources, The Biotechnology Centre, University of Yaounde I, P.O. Box 3851, Messa-Yaounde, Yaounde, Cameroon. boudjeko@yahoo.com.
Anantharaju HasithaDrug Discovery Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science-Pilani, Hyderabad campus, Jawahar Nagar, Hyderabad, Andhra Pradesh, -500078, India.
Matharasala GangadharDrug Discovery Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science-Pilani, Hyderabad campus, Jawahar Nagar, Hyderabad, Andhra Pradesh, -500078, India.
Wilfred Fon MbachamDepartment of Biochemistry, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.
Perumal YogeeswariDrug Discovery Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science-Pilani, Hyderabad campus, Jawahar Nagar, Hyderabad, Andhra Pradesh, -500078, India.
Birla Institute of Technology and Science, Pilani · INUniversité de Yaoundé I · CM

Funding

NAM S&T Centre RTF-DCS 14-15
6 · The paper itself

Abstract

backgroundKhaya grandifoliola (C.D.C.) stem bark, Cymbopogon citratus (Stapf) and Cryptolepis sanguinolenta (Lindl.) Schltr leaves are used in Cameroonian traditional medicine for the treatment of inflammatory diseases. Several studies have been performed on the biological activities of secondary metabolites extracted from these plants. However, to the best of our knowledge, the anti-neuro inflammatory and protective roles of the polysaccharides of these three plants have not yet been elucidated. This study aimed at investigating potential use of K. grandifoliola, C. sanguinolenta and C. citratus polysaccharides in the prevention of chronic inflammation.

methodsFirstly, the composition of polysaccharide fractions isolated from K. grandifoliola stem bark (KGF), C. sanguinolenta (CSF) and C. citratus (CCF) leaves was assessed. Secondly, the cytotoxicity was evaluated on Raw 264.7 macrophages and U87-MG glioblastoma cell lines by the MTT assay. This was followed by the in vitro evaluation of the ability of KGF, CSF and CCF to inhibit lipopolysaccharides (LPS) induced overproduction of various pro-inflammatory mediators (NO, ROS and IL1β, TNFα, IL6, NF-kB cytokines). This was done in Raw 264.7 and U87-MG cells. Finally, the in vitro protective effect of KGF, CSF and CCF against LPS-induced toxicity in the U87-MG cells was evaluated.

resultsCCF was shown to mostly contain sugar and no polyphenol while KGP and CSP contained very few amounts of these metabolites (≤ 2%). The three polysaccharide fractions were non-toxic up to 100 μg.mL

conclusionThese results suggest that the polysaccharides of K. grandifoliola, C. citratus and C. sanguinolenta could be beneficial in preventing/treating neurodegenerative diseases in which neuroinflammation is part of the pathophysiology.

Indexed as

AnimalsAnti-Inflammatory AgentsCell Line, TumorCell SurvivalCryptolepisCymbopogonGlioblastomaHumansLipopolysaccharidesMacrophagesMeliaceaeMiceNeurodegenerative DiseasesNF-kappa BPlant ExtractsPlant LeavesAnti-Inflammatory AgentsLipopolysaccharidesNF-kappa BPlant ExtractsPolysaccharidesTumor Necrosis Factor-alphaAnti-neuroinflammatoryCryptolepis sanguinolentaCymbopogon citratusKhaya grandifoliolaLipopolysaccharidesPlant polysaccharide

Identifiers

PMID29530027
PMCPMC5848566
OpenAlexW2801384641

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.