ArticlePloS one2018
Integrated analysis of transcriptome and proteome changes related to the Ogura cytoplasmic male sterility in cabbage.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Advanced breeding techniques inPlant signaling & behavior · 2026Review
- Identification of metabolic pathways associated with cytoplasmic male sterility in cotton (Gossypium hirsutum L.) using Data-Independent Acquisition-based proteomic profiling.BMC plant biology · 2026Article
- Maize nodal root growth maintenance during water deficit: metabolic acclimation and the role of increased solute deposition in osmotic adjustment.Frontiers in plant science · 2025Article
- Analysis ofInternational journal of molecular sciences · 2024Article
- Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus.Plants (Basel, Switzerland) · 2023Article
- Transcriptomic and Proteomic Analyses of Celery Cytoplasmic Male Sterile Line and Its Maintainer Line.International journal of molecular sciences · 2023Article
- Multi-Omics Methods Applied to Flower Development.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops.International journal of molecular sciences · 2022Review
- Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (International journal of molecular sciences · 2022Article
- Did doubly uniparental inheritance (DUI) of mtDNA originate as a cytoplasmic male sterility (CMS) system?BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines.Plants (Basel, Switzerland) · 2022Article
- Transcript Profiling Analysis and ncRNAs' Identification of Male-Sterile Systems ofFrontiers in plant science · 2022Article
- Transcriptome Profiling Reveals Molecular Changes during Flower Development between Male Sterile and Fertile Chinese Cabbage (Life (Basel, Switzerland) · 2021Article
- Genome-wide comparative transcriptome analysis of the A4-CMS line ICPA 2043 and its maintainer ICPB 2043 during the floral bud development of pigeonpea.Functional & integrative genomics · 2021Article
- A comprehensive integrated transcriptome and metabolome analyses to reveal key genes and essential metabolic pathways involved in CMS in kenaf.Plant cell reports · 2021Article
- Combined Transcriptome and Proteome Analysis of Anthers of AL-type Cytoplasmic Male Sterile Line and Its Maintainer Line Reveals New Insights into Mechanism of Male Sterility in Common Wheat.Frontiers in genetics · 2021Article
- Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile LineFrontiers in plant science · 2021Article
- Transcriptomic and proteomic analyses of a new cytoplasmic male sterile line with a wild Gossypium bickii genetic background.BMC genomics · 2020Article
- Integrative analysis of transcriptomic and proteomic changes related to male sterility inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Comparative transcriptome analysis in Chinese cabbage (Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cabbage (Brassica oleracea L. var. capitata), an important vegetable crop in the Brassicaceae family, is economically important worldwide. In the process of hybrid seed production, Ogura cytoplasmic male sterility (OguCMS), controlled by the mitochondrial gene orf138, has been extensively used for cabbage hybrid production with complete and stable male sterility. To identify the critical genes and pathways involved in the sterility and to better understand the underlying molecular mechanisms, the anther of OguCMS line R2P2CMS and the fertile line R2P2 were used for RNA-seq and iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) proteome analysis. RNA-seq analysis generated 13,037,109 to 13,066,594 SE50-clean reads, from the sterile and fertile lines, which were assembled into 36,890 unigenes. Among them, 1,323 differentially expressed genes (DEGs) were identified, consisting of 307 up- and 1016 down-regulated genes. For ITRAQ analysis, a total of 7,147 unique proteins were identified, and 833 were differentially expressed including 538 up- and 295 down-regulated proteins. These were mainly annotated to the ribosome, spliceosome and mRNA surveillance pathways. Combined transcriptomic and proteomic analyses identified 22 and 70 genes with the same and opposite expression profiles, respectively. Using KEGG analysis of DEGs, gibberellin mediated signaling pathways regulating tapetum programmed cell death and four different pathways involved in sporopollenin synthesis were identified. Secretion and translocation of the sporopollenin precursors were identified, and the key genes participating in these pathways were all significantly down-regulated in R2P2CMS. Light and transmission electron (TE) microscopy revealed fat abnormal tapetum rather than vacuolization and degradation at the tetrad and microspore stages of the OguCMS line. This resulted in the failed deposition of sporopollenin on the pollen resulting in sterility. This study provides a comprehensive understanding of the mechanism underlying OguCMS in cabbage.
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