ArticlePLoS genetics2018
Zinc transporters belonging to the Cation Diffusion Facilitator (CDF) family have complementary roles in transporting zinc out of the cytosol.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Systematic mining and characterization of metal transporter families regulating zinc homeostasis provide insights into metal homeostasis inFrontiers in plant science · 2026Article
- Zinc-Mediated Defenses Against Toxic Heavy Metals and Metalloids: Mechanisms, Immunomodulation, and Therapeutic Relevance.International journal of molecular sciences · 2025Review
- The effects of soil micro-environment on the adaptation of Cynodon dactylon (L.) Persoon in a copper smelter's slag dump.Annals of botany · 2025Article
- Zinc Starvation Induces Cell Wall Remodeling and Activates the Antioxidant Defense System inJournal of fungi (Basel, Switzerland) · 2024Article
- CDF family of zinc transporters ZRC-1, MSC-2, and ZRG-17 are involved in survival at high zinc conditions, vegetative development, and cellulase utilization inJournal of biosciences · 2024Article
- Metalation and activation of ZnBiophysics reviews · 2023Review
- Nutritional immunity: targeting fungal zinc homeostasis.Heliyon · 2021Review
- Zinc toxicity in plants: a review.Planta · 2021Review
- Downregulation of Zn-transporters along with Fe and redox imbalance causes growth and photosynthetic disturbance in Zn-deficient tomato.Scientific reports · 2021Article
- Zinc at the Host-Fungus Interface: How to Uptake the Metal?Journal of fungi (Basel, Switzerland) · 2020Review
- Chromatographic detection of low-molecular-mass metal complexes in the cytosol of Saccharomyces cerevisiae.Metallomics : integrated biometal science · 2020Article
- Detailed analyses of the crucial functions of Zn transporter proteins in alkaline phosphatase activation.The Journal of biological chemistry · 2020Article
- Zinc homeostasis in the secretory pathway in yeast.Current opinion in chemical biology · 2020Review
- Transcription factors and transporters in zinc homeostasis: lessons learned from fungi.Critical reviews in biochemistry and molecular biology · 2020Review
- The intersection of host and fungus through the zinc lens.Current opinion in microbiology · 2019Review
- The Loz1 transcription factor from Schizosaccharomyces pombe binds to Loz1 response elements and represses gene expression when zinc is in excess.Molecular microbiology · 2019Article
- FRET Microscopy in Yeast.Biosensors · 2019Review
- Zinc-dependent activation of the Pho8 alkaline phosphatase inThe Journal of biological chemistry · 2019Article
- MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development inFrontiers in plant science · 2018Article
- Zinc transporters and their functional integration in mammalian cells.The Journal of biological chemistryReview
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Zinc is an essential trace element that is required for the function of a large number of proteins. As these zinc-binding proteins are found within the cytosol and organelles, all eukaryotes require mechanisms to ensure that zinc is delivered to organelles, even under conditions of zinc deficiency. Although many zinc transporters belonging to the Cation Diffusion Facilitator (CDF) families have well characterized roles in transporting zinc into the lumens of intracellular compartments, relatively little is known about the mechanisms that maintain organelle zinc homeostasis. The fission yeast Schizosaccharomyces pombe is a useful model system to study organelle zinc homeostasis as it expresses three CDF family members that transport zinc out of the cytosol into intracellular compartments: Zhf1, Cis4, and Zrg17. Zhf1 transports zinc into the endoplasmic reticulum, and Cis4 and Zrg17 form a heterodimeric complex that transports zinc into the cis-Golgi. Here we have used the high and low affinity ZapCY zinc-responsive FRET sensors to examine cytosolic zinc levels in yeast mutants that lack each of these CDF proteins. We find that deletion of cis4 or zrg17 leads to higher levels of zinc accumulating in the cytosol under conditions of zinc deficiency, whereas deletion of zhf1 results in zinc accumulating in the cytosol when zinc is not limiting. We also show that the expression of cis4, zrg17, and zhf1 is independent of cellular zinc status. Taken together our results suggest that the Cis4/Zrg17 complex is necessary for zinc transport out of the cytosol under conditions of zinc-deficiency, while Zhf1 plays the dominant role in removing zinc from the cytosol when labile zinc is present. We propose that the properties and/or activities of individual CDF family members are fine-tuned to enable cells to control the flux of zinc out of the cytosol over a broad range of environmental zinc stress.
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