ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2018
Comparative analysis of Campylobacter isolates from wild birds and chickens using MALDI-TOF MS, biochemical testing, and DNA sequencing.
Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Are we overestimating risk of enteric pathogen spillover from wild birds to humans?Biological reviews of the Cambridge Philosophical Society · 2020Pooled it
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- Evaluation of the Application of PCR and MALDI-TOF MS Methods for the Identification ofPathogens (Basel, Switzerland) · 2026Article
- Beyond AMR and virulence databases: genome wide associations of closely relatedFrontiers in microbiology · 2026Article
- Antimicrobial resistance ofApplied and environmental microbiology · 2025Article
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- Antimicrobial Resistance Genes in Respiratory Bacteria from Weaned Dairy Heifers.Pathogens (Basel, Switzerland) · 2024Article
- A Systematic Review on the Role of Wildlife as Carriers and Spreaders ofAnimals : an open access journal from MDPI · 2023Review
- Occurrence ofFoods (Basel, Switzerland) · 2023Article
- Prevalence, genomic characterization and antimicrobial resistance ofFrontiers in microbiology · 2023Article
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- Analyses of publicly availableVirulence · 2021Article
- Investigation of MALDI-TOF Mass Spectrometry for Assessing the Molecular Diversity ofDiagnostics (Basel, Switzerland) · 2021Article
- Genomic Characterization ofFrontiers in cellular and infection microbiology · 2021Article
- Review
- Characterization of Campylobacter jejuni, Campylobacter upsaliensis, and a novel Campylobacter sp. in a captive non-human primate zoological collection.Journal of medical primatology · 2019Article
- Application of MALDI Biotyper System for Rapid Identification of Bacteria Isolated from a Fresh Produce Market.Current microbiology · 2019Article
- Developments in Rapid Detection Methods for the Detection of FoodborneFrontiers in microbiology · 2018Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compared to conventional biochemical testing methods and nucleic acid analyses (16S rDNA sequencing, hippurate hydrolysis gene testing, whole genome sequencing [WGS]) for species identification of Campylobacter isolates obtained from chickens ( Gallus gallus domesticus, n = 8), American crows ( Corvus brachyrhynchos, n = 17), a mallard duck ( Anas platyrhynchos, n = 1), and a western scrub-jay ( Aphelocoma californica, n = 1). The test results for all 27 isolates were in 100% agreement between MALDI-TOF MS, the combined results of 16S rDNA sequencing, and the hippurate hydrolysis gene PCR ( p = 0.0027, kappa = 1). Likewise, the identifications derived from WGS from a subset of 14 isolates were in 100% agreement with the MALDI-TOF MS identification. In contrast, biochemical testing misclassified 5 isolates of C. jejuni as C. coli, and 16S rDNA sequencing alone was not able to differentiate between C. coli and C. jejuni for 11 sequences ( p = 0.1573, kappa = 0.0857) when compared to MALDI-TOF MS and WGS. No agreement was observed between MALDI-TOF MS dendrograms and the phylogenetic relationships revealed by rDNA sequencing or WGS. Our results confirm that MALDI-TOF MS is a fast and reliable method for identifying Campylobacter isolates to the species level from wild birds and chickens, but not for elucidating phylogenetic relationships among Campylobacter isolates.
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