ArticleAmerican journal of human biology : the official journal of the Human Biology Council2018
A novel pooled-sample multiplex luminex assay for high-throughput measurement of relative telomere length.
Article in American journal of human biology : the official journal of the Human Biology Council, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Maternal and early-life arsenic exposure and relative telomere length in children: Findings from the BiRCH cohort.Environmental research · 2026Article
- Determinants of chromosome-specific telomere lengths among 2573 All of Us participants.Nature communications · 2026Article
- Exposure to metal mixtures and telomere length in Bangladeshi children.American journal of epidemiology · 2025Article
- High-throughput single telomere analysis using DNA microarray and fluorescent in situ hybridization.Nucleic acids research · 2024Article
- A Transcriptome and Methylome Study Comparing Tissues of Early and Late Onset Colorectal Carcinoma.International journal of molecular sciences · 2022Article
- Article
- Urinary arsenic and relative telomere length in 5-7 year old children in Bangladesh.Environment international · 2021Article
- Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.PloS one · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectivesRelative telomere length (RTL) is a potential biomarker of aging and risk for chronic disease. Previously, we developed a probe-based RTL assay on Luminex platform, where probes for Telomere (T) and reference gene (R) for a given DNA sample were tested in a single well. Here, we describe a method of pooling multiple samples in one well to increase the throughput and cost-effectiveness.
methodsWe used four different microbeads for the same T-probe and four different microbeads for the same R-probe. Each pair of probe sets were hybridized to DNA in separate plates and then pooled in a single plate for all the subsequent steps. We used DNA samples from 60 independent individuals and repeated in multiple batches to test the precision.
resultsThe precision was good to excellent with Intraclass correlation coefficient (ICC) of 0.908 (95% CI 0.856-0.942). More than 67% of the variation in the RTL could be explained by sample-to-sample variation; less than 0.1% variation was due to batch-to-batch variation and 0.3% variation was explained by bead-to-bead variation. We increased the throughput of RTL Luminex assay from 60 to 240 samples per run. The new assay was validated against the original Luminex assay without pooling (r = 0.79, P = 1.44 × 10
conclusionWe describe a novel high throughput pooled-sample multiplex Luminex assay for RTL with good to excellent precision suitable for large-scale studies.
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Registered trials
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