Evidence map›Paper›PMID 29527233›Full record

ArticlePlant methods2018

Improved apple latent spherical virus-induced gene silencing in multiple soybean genotypes through direct inoculation of agro-infiltrated

C R Gedling, E M Ali, A Gunadi, J J Finer, K Xie, Y Liu, N Yoshikawa, F Qu, A E Dorrance

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

C R Gedling *1Department of Plant Pathology, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 USA.
E M Ali *1Department of Plant Pathology, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 USA.
A Gunadi2Department of Horticulture and Crop Science, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 USA.
J J Finer2Department of Horticulture and Crop Science, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 USA.
K Xie3School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Y Liu4MOE Key Laboratory of Bioinformatics, Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084 China.
N Yoshikawa5Plant Pathology Lab, Facility of Agriculture, Iwate University, Morioka, Japan.
F Qu1Department of Plant Pathology, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 USA.
A E Dorrance1Department of Plant Pathology, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 USA.ORCID 0000-0003-4138-6707
The Ohio State University · USCenter for Life Sciences · CNIwate University · JPTree Fruit Research Laboratory · USUniversity of Science and Technology Beijing · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundVirus induced gene silencing (VIGS) is a powerful genomics tool for interrogating the function of plant genes. Unfortunately, VIGS vectors often produce disease symptoms that interfere with the silencing phenotypes of target genes, or are frequently ineffective in certain plant genotypes or tissue types. This is especially true in crop plants like soybean [

resultsIn this report, the ALSV VIGS vector was modified by mobilizing ALSV cDNAs into a binary vector compatible with

conclusionsThis modified protocol allowed for inoculation of soybean plants via simple mechanical rubbing with the homogenate of

Indexed as

Apple latent spherical virusSoybean functional analysisVIGS

Identifiers

PMID29527233
PMCPMC5838930
OpenAlexW2797491950

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.