ReviewExperimental eye research2018
Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.
Open access · greenAbstract readReviewConsensus Statement
In one paragraph
Review in Experimental eye research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 260 papers.
0numbers the graph read from it
0cells of the map it votes in
260citing papers in PubMed
26.0field-weighted citation impact, top 1% of its field
What it found
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Who cites it
260 citing papers in PubMed, 338 citations in OpenAlex.
- Nuclear LC3 Interactome Profiling Identifies Clathrin Heavy Chain as a Mediator of Nuclear LC3 Translocation in Trabecular Meshwork Cells.bioRxiv : the preprint server for biology · 2026Article
- Author Response: Human Trabecular Meshwork and Hepatocyte Growth Factor.Investigative ophthalmology & visual science · 2026Article
- MicroRNA profiling of filtering and non-filtering regions of human trabecular meshwork: changes with ageing and in glaucoma.Molecular biology reports · 2026Article
- Investigating the coupled effects of stiffness and stretch on the trabecular meshwork cells using a hydrogel-integrated microfluidic system.Biomaterials science · 2026Article
- Human Trabecular Meshwork and Hepatocyte Growth Factor.Investigative ophthalmology & visual science · 2026Article
- Integrin-Linked Kinase Suppression Reduces SPARC's Effect on Intraocular Pressure and Extracellular Matrix.Investigative ophthalmology & visual science · 2026Article
- Composition Matters: Collagen vs Polyacrylamide Modulates Distinct Trabecular Meshwork Cell Traction.ACS biomaterials science & engineering · 2026Article
- A Primary Open-Angle Glaucoma Locus Near Transcription FactorOphthalmology science · 2026Article
- Cross-linked actin networks (CLANs) and their role in the trabecular meshwork.Experimental eye research · 2026Review
- Novel Role for CGRP in Aqueous Humor Outflow.Investigative ophthalmology & visual science · 2026Article
- Segmental Mechanobiology of Normal and Glaucomatous Human Trabecular Meshwork Cells.ACS measurement science au · 2026Article
- Article
- Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma.Investigative ophthalmology & visual science · 2026Article
- TXNDC5 in POAG: Promoting Extracellular Matrix Protein Accumulation and Raising Intraocular Pressure.Investigative ophthalmology & visual science · 2026Article
- Variant-to-gene mapping identifiesbioRxiv : the preprint server for biology · 2026Article
- Isobaric quantitative proteomics reveals altered extracellular matrix, cytoskeletal, and degradation pathways in glaucomatous trabecular meshwork cells.Scientific reports · 2026Article
- Chromatin dynamics identifies 78 genes at loci associated with elevated intraocular pressure and primary open-angle glaucoma.bioRxiv : the preprint server for biology · 2026Article
- Left-Right Determination Factor 2 (LEFTY2) Is an Aqueous Humor Biomarker for Exfoliation Glaucoma.Translational vision science & technology · 2026Article
- Tissue-Specific Antioxidant Capacity in Primary Open-Angle Glaucoma Patients.Investigative ophthalmology & visual science · 2026Article
- Pro-Homeostatic Effects of Estrogen on Intraocular Pressure and the Trabecular Meshwork.Investigative ophthalmology & visual science · 2026Article
200 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
57 authors at 20 institutions in 3 countries.
Kate E KellerOregon Health and Science University, United States.
Sanjoy K BhattacharyaUniversity of Miami, United States.
Theresa BorrásUniversity of North Carolina-Chapel Hill, United States.
Thomas M BrunnerGlaucoma Research Foundation, United States.
Sunee ChansangpetchUniversity of California, San Francisco, United States.
Abbott F ClarkUniversity of North Texas Health Sciences Center, United States.
W Michael DismukeAerie Pharmaceuticals, United States.
Yiqin DuUniversity of Pittsburgh, United States.
Michael H ElliottUniversity of Oklahoma, United States.
C Ross EthierGeorgia Institute of Technology, United States.
Jennifer A FaralliUniversity of Wisconsin, United States.
Thomas F FreddoMassachusetts College of Pharmacy and Health Sciences, United States.
Rudolf FuchshoferUniversity of Regensburg, Germany.
Michael GiovingoJohn H. Stroger, Jr. Hospital of Cook County, United States.
Haiyan GongBoston University, United States.
Pedro GonzalezDuke University, United States.
Alex HuangUniversity of California, Los Angeles, United States.
Murray A JohnstoneUniversity of Washington, United States.
Paul L KaufmanUniversity of Wisconsin, United States.
Mary J KelleyOregon Health and Science University, United States.
Paul A KnepperUniversity of Illinois at Chicago, United States.
Casey C KopczynskiAerie Pharmaceuticals, United States.
John G KuchteyVanderbilt University, United States.
Rachel W KuchteyVanderbilt University, United States.
Markus H KuehnUniversity of Iowa, United States.
Raquel L LiebermanGeorgia Institute of Technology, United States.
Shan C LinUniversity of California, San Francisco, United States.
Paloma LitonDuke University, United States.
Yutao LiuAugusta University, United States.
Elke Lütjen-DrecollUniversity of Erlangen-Nuremberg, Germany.
Weiming MaoUniversity of North Texas Health Sciences Center, United States.
Marisse Masis-SolanoUniversity of California, San Francisco, United States.
Fiona McDonnellDuke University, United States.
Colleen M McDowellUniversity of North Texas Health Sciences Center, United States.
Darryl R OverbyImperial College, London, United Kingdom.
Padmanabhan P PattabiramanCase Western Reserve University, United States.
Vijay K RaghunathanUniversity of Houston, United States.
P Vasanth RaoDuke University, United States.
Douglas J RheeCase Western Reserve University, United States.
Uttio Roy ChowdhuryMayo Clinic, United States.
Paul RussellUniversity of California, Davis, United States.
John R SamplesWestern Glaucoma Foundation, United States.
Donald SchwartzEyeSonix, United States.
Evan B StubbsLoyola University, Chicago, United States.
Ernst R TammUniversity of Regensburg, Germany.
James C TanUniversity of Southern California, United States.
Carol B TorisCase Western Reserve University, United States.
Karen Y TorrejonGlauconix, United States.
Janice A VrankaOregon Health and Science University, United States.
Mary K WirtzOregon Health and Science University, United States.
Thomas YorioUniversity of North Texas Health Sciences Center, United States.
Jie ZhangUniversity of California, Los Angeles, United States.
Gulab S ZodeUniversity of North Texas Health Sciences Center, United States.
Michael P FautschDepartment of Ophthalmology, Mayo Clinic, 200 1st St SW, Rochester, MN 55905, United States. Electronic address: fautsch.michael@mayo.edu.
Donna M PetersDepartment of Pathology & Laboratory Medicine, University of Wisconsin, 1300 University Ave, Madison, WI 53706, United States. Electronic address: dmpeter2@wisc.edu.
Ted S AcottDepartment of Ophthalmology, Department of Biochemistry & Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, United States. Electronic address: acott@ohsu.edu.
W Daniel StamerDepartment of Ophthalmology, Duke University, DUMC 3802, Durham, NC 27705, United States. Electronic address: dan.stamer@duke.edu.
Duke University · USOregon Health & Science University · USUniversity of North Texas · USCase Western Reserve University · USUniversity of California, San Francisco · USAerie Pharmaceuticals (United States) · USGeorgia Institute of Technology · USGlaucoma Research Foundation · USUniversity of California, Los Angeles · USUniversity of Regensburg · DEVanderbilt University · USAugusta University Health · USEyeSonix (United States) · USFriedrich-Alexander-Universität Erlangen-Nürnberg · DEGlauconix (United States) · USImperial College London · GBJohn H. Stroger, Jr. Hospital of Cook County · USLoyola University Chicago · USMassachusetts College of Pharmacy and Health Sciences · USMayo Clinic · US
Funding
Viral VectorP30CA086862 · NCI · UNIVERSITY OF IOWA · PI Christopher K Laubenthal · 2000 to 2026
$70.0MVISION RESEARCHP30EY005722 · NEI · DUKE UNIVERSITY · PI Vadim Y Arshavsky · 1985 to 2026
$19.3MVision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Xian-Jie Yang · 1985 to 2026
$16.8MUW Vision Research Core - Administrative CoreP30EY016665 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI Donna M Peters · 2005 to 2026
$12.6MP30-CENTER CORE GRANT FOR VISION RESEARCHP30EY021725 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI Michelle C Callegan, MICHAEL H ELLIOTT · 2011 to 2026
$10.5MRequest for Supplement to Promote Diversity in Health Related ResearchR01EY021205 · NEI · GEORGIA INSTITUTE OF TECHNOLOGY · PI Raquel L Lieberman · 2011 to 2026
$6.8MControl of Trabecular Meshwork CytoskeletonR01EY017006 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI PETERS, DONNA M · 2006 to 2024
$6.1MREGULATION OF OUTFLOW FACILITY BY GENE TRANSFERR01EY011906 · NEI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI BORRAS, TERESA · 1997 to 2016
$5.6MExtracellular Matrix and Outflow ResistanceR01EY019643 · NEI · OREGON HEALTH & SCIENCE UNIVERSITY · PI KELLER, KATE E · 2010 to 2025
$5.0MAutophagy and Mechanotransduction in the Trabecular MeshworkR01EY026885 · NEI · DUKE UNIVERSITY · PI Paloma Liton · 2016 to 2026
$4.7MMechanisms of Trabecular Meshwork Regeneration by Stem CellsR01EY025643 · NEI · UNIVERSITY OF SOUTH FLORIDA · PI Yiqin Du · 2015 to 2026
$4.2MCrosstalk Between Unfolded Protein Response and Autophagy for the Treatment of GlaucomaR01EY026177 · NEI · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI ZODE, GULAB · 2016 to 2025
$3.8MBLRD VA I01 BX003938NEI NIH HHS K08 EY024674NEI NIH HHS K99 EY022077NEI NIH HHS P30 EY000331NEI NIH HHS P30 EY005722NEI NIH HHS P30 EY016665NEI NIH HHS P30 EY021725NEI NIH HHS R00 EY022077NEI NIH HHS R01 EY011906NEI NIH HHS R01 EY017006NEI NIH HHS R01 EY019643NEI NIH HHS R01 EY021205NEI NIH HHS R01 EY022634NEI NIH HHS R01 EY025643NEI NIH HHS R01 EY026009NEI NIH HHS R01 EY026048NEI NIH HHS R01 EY026177NEI NIH HHS R01 EY026220NEI NIH HHS R01 EY026529NEI NIH HHS R01 EY026885NEI NIH HHS R01 EY026935NEI NIH HHS R01 EY027733NEI NIH HHS R21 EY027079NEI NIH HHS R21 EY028671NIGMS NIH HHS R01 GM100909RRD VA I01 RX001163
Abstract
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
Indexed as
Cell Culture TechniquesGuidelines as TopicAge FactorsAnimalsBiomarkersCell SeparationFetusHumansTissue and Organ HarvestingTissue DonorsTissue PreservationTrabecular MeshworkBiomarkersAqueous humor dynamicsConventional outflowGlaucomaIntraocular pressureOcular hypertension
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