Evidence map›Paper›PMID 29526772›Full record

ArticleInsect biochemistry and molecular biology2018

A deep insight into the male and female sialotranscriptome of adult Culex tarsalis mosquitoes.

José M C Ribeiro, Ines Martin-Martin, Fernando R Moreira, Kristen A Bernard, Eric Calvo

Abstract read
In one paragraph

Article in Insect biochemistry and molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Intrinsic factors driving mosquito vector competence and viral evolution: a review.Frontiers in cellular and infection microbiology · 2023
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. A Salivary Odorant-Binding Protein MediatesInternational journal of molecular sciences · 2021
    Article
  9. Article
  10. Article
  11. The structures of two salivary proteins from the West Nile vectorCurrent research in structural biology · 2021
    Article
  12. Biomolecules · 2020
    Article
  13. Article
  14. Identification of NovelInternational journal of molecular sciences · 2020
    Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

José M C RibeiroSection of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, 12735 Twinbrook Parkway Room 2E32D, Rockville, MD, 20852, United States. Electronic address: jribeiro@niaid.nih.gov.
Ines Martin-MartinSection of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, 12735 Twinbrook Parkway Room 2E32D, Rockville, MD, 20852, United States.
Fernando R MoreiraDepartment of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Dr., Madison, WI, 53706, United States.
Kristen A BernardDepartment of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Dr., Madison, WI, 53706, United States.
Eric CalvoSection of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, 12735 Twinbrook Parkway Room 2E32D, Rockville, MD, 20852, United States.

Funding

Vector-Borne Diseases: Biology Of Vector Host RelationshipZIAAI000810 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI RIBEIRO, JOSE · 2009 to 2025
$20.8M
Arthropod saliva in vector-borne disease transmission: Functional analysis of novel domains in salivary secretionsZIAAI001246 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI CALVO, ERIC · 2019 to 2025
$10.0M
Parasitology and Vector Biology Training ProgramT32AI007414 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI Lyric Colleen Bartholomay · 1992 to 2026
$4.5M
Mechanisms of Enhanced Arbovirus Infection by Mosquito SalivaR01AI099231 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI BERNARD, KRISTEN A · 2013 to 2016
$1.5M
Mosquito-Virus-Host Interactions and WNV PathogenesisR01CI000232 · CI · WADSWORTH CENTER · PI BERNARD, KRISTEN A · 2005 to 2007
$400k
Intramural NIH HHS ZIA AI000810NCPDCID CDC HHS R01 CI000232NIAID NIH HHS R01 AI099231NIAID NIH HHS T32 AI007414
6 · The paper itself

Abstract

Previously, a Sanger-based sialotranscriptome analysis of adult female Culex tarsalis was published based on ∼2000 ESTs. During the elapsed 7.5 years, pyrosequencing has been discontinued and Illumina sequences have increased considerable in size and decreased in price. We here report an Illumina-based sialotranscriptome that allowed finding the missing apyrase from the salivary transcriptome of C. tarsalis, to determine several full-length members of the 34-62 kDa family, when a single EST has been found previously, in addition to identifying many salivary families with lower expression levels that were not detected previously. The use of multiple libraries including salivary glands and carcasses from male and female organisms allowed for an unprecedented insight into the tissue specificity of transcripts, and in this particular case permitting identification of transcripts putatively associated with blood feeding, when exclusive of female salivary glands, or associated with sugar feeding, when transcripts are found upregulated in both male and female glands.

Indexed as

Sex CharacteristicsAnimalsCulexFemaleGene Expression ProfilingInsect ProteinsMaleSialoglycoproteinsInsect ProteinsSialoglycoproteinsBlood-feedingMosquitoesSalivary glandsTranscriptome

Identifiers

PMID29526772
PMCPMC5927831

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.