Evidence map›Paper›PMID 29526770›Full record

ArticleInsect biochemistry and molecular biology2018

HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.

Dujuan Dong, Yang Zhang, Vlastimil Smykal, Lin Ling, Alexander S Raikhel

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect biochemistry and molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Genomic and insulin-mediated control of metabolic homeostasis by the mosquito ecdysone-induced gene E93.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Article
  6. Mosquito gene targeted RNAi studies for vector control.Functional & integrative genomics · 2023
    Review
  7. The buzz in the field: the interaction between viruses, mosquitoes, and metabolism.Frontiers in cellular and infection microbiology · 2023
    Review
  8. Article
  9. Review
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Dujuan DongDepartment of Entomology, University of California, Riverside, CA 92521, USA; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan 250100, China.
Yang ZhangDepartment of Entomology, University of California, Riverside, CA 92521, USA; Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Vlastimil SmykalDepartment of Entomology, University of California, Riverside, CA 92521, USA.
Lin LingDepartment of Entomology, University of California, Riverside, CA 92521, USA.
Alexander S RaikhelDepartment of Entomology, University of California, Riverside, CA 92521, USA; Institute for Integrative Genomic Biology, University of California, Riverside, CA 92521, USA. Electronic address: alexander.raikhel@ucr.edu.
University of California, Riverside · USChinese Academy of Sciences · CNShandong University · CN

Funding

MOLECULAR BASIS OF ECDYSTEROID ACTION IN THE MOSQUITOR01AI036959 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI RAIKHEL, ALEXANDER SIMEON, ROY, SOURAV · 1995 to 2022
$10.0M
NIAID NIH HHS R01 AI036959
6 · The paper itself

Abstract

The Aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. Carbohydrate metabolism (CM) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. The steroid hormone 20-hydroxyecdysone (20E) and the ecdysone receptor (EcR) are important regulators of CM, coordinating it with female reproductive events. We report here that the NR4A nuclear receptor AHR38 plays a critical role in mediating these actions of 20E and EcR. AHR38 RNA interference (RNAi) depletion in female mosquitoes blocked the transcriptional activation of CM genes encoding phosphoglucomutase (PGM) and trehalose-6-phophate synthase (TPS); it caused an increase of glycogen accumulation and a decrease of the circulating sugar trehalose. This treatment also resulted in a dramatic reduction in fecundity. Considering that these phenotypes resulting from AHR38 RNAi depletion are similar to those of EcR RNAi, we investigated a possible connection between these transcription factors in CM regulation. EcR RNAi inhibits the AHR38 gene expression. Moreover, the 20E-induced EcR complex directly activates AHR38 by binding to the ecdysone response element (EcRE) in the upstream regulatory region of this gene. The present work has implicated AHR38 in the 20E-mediated control of CM and provided new insight into mechanisms of 20E regulation of metabolism during female mosquito reproduction.

Indexed as

AedesAnimalsCarbohydrate MetabolismEcdysteroneFemaleInsect ProteinsMaleNuclear Receptor Subfamily 4, Group A, Member 1ReproductionEcdysteroneInsect ProteinsNuclear Receptor Subfamily 4, Group A, Member 1Disease vectorHormoneMetabolismMosquitoNuclear receptor

Identifiers

PMID29526770
PMCPMC5959765
OpenAlexW2791175685

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.