ArticleCurrent biology : CB2018
The Arabidopsis SPIRAL2 Protein Targets and Stabilizes Microtubule Minus Ends.
Article in Current biology : CB, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Technological advances in visualizing and rewiring microtubules during plant development.Journal of experimental botany · 2025Review
- Microtubule Minus-End Binding Proteins in Cancer: Advances.Diagnostics (Basel, Switzerland) · 2025Review
- Transverse cortical microtubule arrays form persistent unipolar domains in hypocotyl cells ofMolecular biology of the cell · 2025Article
- Fibrous network nature of plant cell walls enables tunable mechanics for development.Nature communications · 2025Article
- Regulation of cytoskeleton dynamics and its interplay with force in plant cells.Biophysics reviews · 2024Review
- A divergent tumor overexpressed gene domain and oligomerization contribute to SPIRAL2 function in stabilizing microtubule minus ends.The Plant cell · 2024Article
- TOGging up microtubule ends: The plant-specific microtubule minus-end tracking SPR2 has a unique TOG domain.The Plant cell · 2024Article
- New insights into the functions and regulations of MAP215/MOR1 and katanin, two conserved microtubule-associated proteins in Arabidopsis.Plant signaling & behavior · 2023Article
- Cellulose assembles into helical bundles of uniform handedness in cell walls with abnormal pectin composition.The Plant journal : for cell and molecular biology · 2023Article
- Anatomical observation and transcriptome analysis of branch-twisted mutations in Chinese jujube.BMC genomics · 2023Article
- The cell biology of primary cell walls during salt stress.The Plant cell · 2023Review
- Crystal structure of the C-terminal domain of the plant-specific microtubule-associated protein Spiral2.Acta crystallographica. Section F, Structural biology communications · 2023Article
- Crystal structure of the Arabidopsis SPIRAL2 C-terminal domain reveals a p80-Katanin-like domain.PloS one · 2023Article
- MOR1/MAP215 acts synergistically with katanin to control cell division and anisotropic cell elongation in Arabidopsis.The Plant cell · 2022Article
- Finding a right place to cut: How katanin is targeted to cellular severing sites.Quantitative plant biology · 2022Review
- Microtubule Organization Is Essential for Maintaining Cellular Morphology and Function.Oxidative medicine and cellular longevity · 2022Review
- Crosstalk between basal extracellular matrix adhesion and building of apical architecture during morphogenesis.Biology open · 2021Review
- FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.PLoS biology · 2021Article
- Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes.Trends in cell biology · 2021Review
- Article
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3 authors.
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Abstract
The contribution of microtubule tip dynamics to the assembly and function of plant microtubule arrays remains poorly understood. Here, we report that the Arabidopsis SPIRAL2 (SPR2) protein modulates the dynamics of the acentrosomal cortical microtubule plus and minus ends in an opposing manner. Live imaging of a functional SPR2-mRuby fusion protein revealed that SPR2 shows both microtubule plus- and minus-end tracking activity in addition to localization at microtubule intersections and along the lattice. Analysis of microtubule dynamics showed that cortical microtubule plus ends rarely undergo catastrophe in the spr2-2 knockout mutant compared to wild-type. In contrast, cortical microtubule minus ends in spr2-2 depolymerized at a much faster rate than in wild-type. Destabilization of the minus ends in spr2-2 caused a significant decrease in the lifetime of microtubule crossovers, which dramatically reduced the microtubule-severing frequency and inhibited light-induced microtubule array reorientation. Using in vitro reconstitution experiments combined with single-molecule imaging, we found that recombinant SPR2-GFP intrinsically localizes to microtubule minus ends, where it binds stably and inhibits their dynamics. Together, our data establish SPR2 as a new type of microtubule tip regulator that governs the length and lifetime of microtubules.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.