ArticleMethods in molecular biology (Clifton, N.J.)2018
Directional Collective Migration in Wound Healing Assays.
Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Macrophage- or neutrophil-expressed UPP1 drives tumor growth and immune suppression in the tumor microenvironment.Science advances · 2026Article
- Bioactive derivatives of the antimicrobial peptide esculentin-1a promote human dermal fibroblast migration and activate genes involved in early wound healing.BBA advances · 2026Article
- Development of Novel ROCK Inhibitors via 3D-QSAR and Molecular Docking Studies: A Framework for Multi-Target Drug Design.Pharmaceutics · 2024Article
- Evaluating the Effects of BSA-Coated Gold Nanorods on Cell Migration Potential and Inflammatory Mediators in Human Dermal Fibroblasts.Journal of functional biomaterials · 2024Article
- Cellular and molecular mechanisms of skin wound healing.Nature reviews. Molecular cell biology · 2024Review
- A high-precision wound healing assay based on photosensitized culture substrates.Scientific reports · 2024Article
- Photoactivatable surfaces resolve the impact of gravity vector on collective cell migratory characteristics.Science and technology of advanced materials · 2023Article
- A Novel In Vitro Wound Healing Assay Using Free-Standing, Ultra-Thin PDMS Membranes.Membranes · 2022Article
- In Vitro and In Vivo Characterization Methods for Evaluation of Modern Wound Dressings.Pharmaceutics · 2022Review
- Circular gap forming device and two-dimensional area calculation for in vitro cell migration study.Cell and tissue banking · 2022Article
- Multiscale modeling of collective cell migration elucidates the mechanism underlying tumor-stromal interactions in different spatiotemporal scales.Scientific reports · 2022Article
- The role of cell geometry and cell-cell communication in gradient sensing.PLoS computational biology · 2022Article
- EBV-Induced CXCL8 Upregulation Promotes Vasculogenic Mimicry in Gastric CarcinomaFrontiers in cellular and infection microbiology · 2022Article
- Hypoxia-InducedInternational journal of molecular sciences · 2021Article
- Interaction of a Migrating Cell Monolayer with a Flexible Fiber.Biophysical journal · 2021Article
- The Frequent Sampling of Wound Scratch Assay Reveals the "Opportunity" Window for Quantitative Evaluation of Cell Motility-Impeding Drugs.Frontiers in cell and developmental biology · 2021Article
- Recent advances in understanding the role of metabolic heterogeneities in cell migration.Faculty reviews · 2021Review
- Mustard Seed (Molecules (Basel, Switzerland) · 2020Article
- Analysis of Random Migration of Cancer Cells in 3D.Bio-protocol · 2020Article
- Engineering multi-layered tissue constructs using acoustic levitation.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell migration is suppressed by confluence in a process called contact inhibition. Relieving contact inhibition upon scratching is one of the simplest ways to induce cell migration in a variety of cell types. Wound healing is probably most relevant to epithelial monolayers, because epithelial cells generally assume a barrier function, which must be restored as fast as possible by the healing process. This versatile assay, however, can also be applied to fibroblasts and to tumor cell types. Furthermore, assessing the cell response to scratch wounding requires no special equipment or reagents. It is one of the few cell migration assays, which can even be performed without videomicroscopy, since the closure of the wound can be estimated at fixed time points. Several hours after wounding, directional collective migration is easily assessed and quantified. However, cell proliferation, which is also induced by the relief of contact inhibition, is one of the confounding factors of wound healing assays that must be taken into account. A recent alternative to the scratch-induced wound is to use special inserts to seed cells into closely spaced chambers. When the insert is removed, contact inhibition is relieved, similar to the scratch-induced wound. In this chapter, we provide the protocol of the two methods and compare their advantages and disadvantages. We also provide a protocol to estimate cell proliferation upon wound healing based on the incorporation of the nucleotide analog EdU.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.