ArticleJournal of industrial microbiology & biotechnology2018
Production of pikromycin using branched chain amino acid catabolism in Streptomyces venezuelae ATCC 15439.
Article in Journal of industrial microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Inactivation of the sco2730/2731 copper chaperone-transporter system in Streptomyces coelicolor and its orthologs in Streptomyces venezuelae, together with chromosomal end deletion, greatly enhances secondary metabolism.Microbial cell factories · 2026Article
- Branched-Chain Amino Acids Mediate the Regulation of SACE_Lrp on the Aminotransferase IlvE Involved in Erythromycin Biosynthesis in Saccharopolyspora erythraea.Microbial biotechnology · 2026Article
- Characterization of an aminotransferase TlBCAT from Trichoderma longibrachiatum UN32 involved in dendrobine-type total alkaloids biosynthesis.World journal of microbiology & biotechnology · 2024Article
- Improving the production of natamycin inFood science and biotechnology · 2024Article
- Effects of branched-chain amino acids on Shiraia perylenequinone production in mycelium cultures.Microbial cell factories · 2023Article
- Origin of the 3-methylglutaryl moiety in caprazamycin biosynthesis.Microbial cell factories · 2022Article
- Marine Macrolides to Tackle Antimicrobial Resistance ofMarine drugs · 2022Review
- Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production.Applied microbiology and biotechnology · 2022Article
- A novel strategy of gene screen based on multi-omics in Streptomyces roseosporus.Applied microbiology and biotechnology · 2022Article
- WblA, a global regulator of antibiotic biosynthesis in Streptomyces.Journal of industrial microbiology & biotechnology · 2021Review
- Superior production of heavy pamamycin derivatives using a bkdR deletion mutant of Streptomyces albus J1074/R2.Microbial cell factories · 2021Article
- Optimization of tetramycin production in Streptomyces ahygroscopicus S91.Journal of biological engineering · 2021Article
- The Onset of Tacrolimus Biosynthesis inAntibiotics (Basel, Switzerland) · 2020Article
- Synthetic biology enabling access to designer polyketides.Current opinion in chemical biology · 2020Review
- Engineering actinomycetes for biosynthesis of macrolactone polyketides.Microbial cell factories · 2019Review
- Growth and differentiation properties of pikromycin-producing Streptomyces venezuelae ATCC15439.Journal of microbiology (Seoul, Korea) · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Branched chain amino acids (BCAA) are catabolized into various acyl-CoA compounds, which are key precursors used in polyketide productions. Because of that, BCAA catabolism needs fine tuning of flux balances for enhancing the production of polyketide antibiotics. To enhance BCAA catabolism for pikromycin production in Streptomyces venezuelae ATCC 15439, three key enzymes of BCAA catabolism, 3-ketoacyl acyl carrier protein synthase III, acyl-CoA dehydrogenase, and branched chain α-keto acid dehydrogenase (BCDH) were manipulated. BCDH overexpression in the wild type strain resulted in 1.3 fold increase in pikromycin production compared to that of WT, resulting in total 25 mg/L of pikromycin. To further increase pikromycin production, methylmalonyl-CoA mutase linked to succinyl-CoA production was overexpressed along with BCDH. Overexpression of the two enzymes resulted in the highest titer of total macrolide production of 43 mg/L, which was about 2.2 fold increase compared to that of the WT. However, it accumulated and produced dehydroxylated forms of pikromycin and methymycin, including their derivatives as well. It indicated that activities of pikC, P450 monooxygenase, newly became a bottleneck in pikromycin synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.