ArticleAMB Express2018
Post-ruminal effects of rumen-protected methionine supplementation with low protein diet using long-term simulation and in vitro digestibility technique.
Article in AMB Express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 20 citations in OpenAlex.
- Effects of Supplementing Rumen-Protected Lysine and Methionine on Apparent Digestibility, Rumen Fermentation Parameters, and Microbial Profiles in Lactating Dairy Cows Under Different Environmental Conditions.Animals : an open access journal from MDPI · 2025Article
- The rumen microbiome and metabolome profile of Ongole crossbreed cattle fed probiotics and protected amino acids.Tropical animal health and production · 2025Article
- Effects of lipopolysaccharide infusion on feed intake, apparent digestibility, rumen fermentation and microorganisms of young Holstein bulls fed diets with different ratios of lysine and methionine.Frontiers in veterinary science · 2024Article
- Fermented soybean meal modified the rumen microbiome to enhance the yield of milk components in Holstein cows.Applied microbiology and biotechnology · 2022Article
- Applying nanotechnology to increase the rumen protection of amino acids in dairy cows.Scientific reports · 2020Article
- Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics.AMB Express · 2019Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Microbial degradation in the rumen and dietary availability of methionine amino acid have been reported as limiting in dairy ruminants. The aim of the present study was to examine the post-ruminal effects of feeding ruminants different concentrations of rumen-protected methionine (RPM) in low crude protein diets using the long-term rumen simulation method (Rusitec) followed by in vitro abomasum and ileum digestibility technique. The experiment contained four treatment groups: (1) high protein, without RPM supplementation (HP); (2) low protein, without RPM supplementation (LP); (3) low protein supplementation with low RPM (LPLM); and (4) low protein supplementation with high RPM (LPHM) mixed per 20 ± 0.04 g basal diet in every fermenter. The results showed that the LPLM and LPHM groups had significantly higher disappearance of crude protein and neutral detergent fiber in the abomasum and ileum than the HP treatment (P < 0.05) and were the same as the LP group (P > 0.05). The proportions of short-chain fatty acids and total volatile fatty acids in the abomasum and ileum were the same between the LPHM and HP groups (P > 0.05); however, the LPLM group was found to be significantly (P < 0.05) lower than the HP group and similar to the LP group (P > 0.05). Rusitec pH before or after changing feed bags and daily ammonia nitrogen production in the abomasum and ileum were non significantly (P > 0.05) different among all groups. In conclusion, RPM supplementation with low crude protein diets promoted post-ruminal digestibility and production of volatile fatty acids.
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