ArticleScientific reports2018
Identification of new abscisic acid receptor agonists using a wheat cell-free based drug screening system.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- DELLA proteins interact with HAC1 and control plant growth via transcriptional regulation of the growth repressor gene ERF2.The Plant cell · 2026Article
- AHG1-AFP interaction as a regulatory node in the ABA response during seed germination.The Plant journal : for cell and molecular biology · 2026Article
- Comparative proteomic profiling of receptor kinase signaling reveals key trafficking components enforcing plant stomatal development.Science advances · 2026Article
- Retrieval from vacuolar and endosomal compartments underpinning the neofunctionalization of SNARE in plants.Nature plants · 2025Article
- Comparative Proteomic Profiling of Receptor Kinase Signaling Reveals Key Trafficking Components Enforcing Plant Stomatal Development.bioRxiv : the preprint server for biology · 2025Article
- Deciphering highly potent natural abscisic acid agonists for binding to pyrabactin resistance 1 receptor through computational approaches.Scientific reports · 2025Article
- Half a Century of Progress: The Evolution of Wheat Germ-Based In Vitro Translation into a Versatile Protein Production Method.International journal of molecular sciences · 2025Review
- Chemical application improves stress resilience in plants.Plant molecular biology · 2025Review
- Applications of the wheat germ cell-free protein synthesis system in plant biochemical studies.Plant biotechnology (Tokyo, Japan) · 2024Article
- Optimizing ABA-based chemically induced proximity for enhanced intracellular transcriptional activation and modification response to ABA.Science China. Life sciences · 2024Article
- From Regulation to Application: The Role of Abscisic Acid in Seed and Fruit Development and Agronomic Production Strategies.International journal of molecular sciences · 2024Review
- Small chemical molecules regulating the phytohormone signalling alter the plant's physiological processes to improve stress adaptation, growth and productivity.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Somatic embryogenesis from mature sorghum seeds: An underutilized genome editing recipient system.Heliyon · 2024Article
- GATS tag system is compatible with biotin labelling methods for protein analysis.Scientific reports · 2023Article
- A Potential ABA Analog to Increase Drought Tolerance inInternational journal of molecular sciences · 2023Article
- Identification of a new gibberellin receptor agonist, diphegaractin, by a cell-free chemical screening system.Communications biology · 2023Article
- Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought.Science advances · 2023Article
- A briefly overview of the research progress for the abscisic acid analogues.Frontiers in chemistry · 2022Review
- ABA signalling promotes cell totipotency in the shoot apex of germinating embryos.Journal of experimental botany · 2021Article
- Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology.Frontiers in molecular biosciences · 2021Review
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Authors and funding
8 authors.
Funding
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Abstract
Abscisic acid (ABA) is the main phytohormone involved in abiotic stress response and its adaptation, and is a candidate agrichemical. Consequently, several agonists of ABA have been developed using the yeast two-hybrid system. Here, we describe a novel cell-free-based drug screening approach for the development and validation of ABA receptor agonists. Biochemical validation of this approach between 14 ABA receptors (PYR/PYL/RCARs) and 7 type 2C-A protein phosphatases (PP2CAs) revealed the same interactions as those of previous proteome data, except for nine new interactions. By chemical screening using this approach, we identified two novel ABA receptor agonists, JFA1 (julolidine and fluorine containing ABA receptor activator 1) and JFA2 as its analog. The results of biochemical validation for this approach and biological analysis suggested that JFA1 and JFA2 inhibit seed germination and cotyledon greening of seedlings by activating PYR1 and PYL1, and that JFA2 enhanced drought tolerance without inhibiting root growth by activating not only PYR1 and PYL1 but also PYL5. Thus, our approach was useful for the development of ABA receptor agonists and their validation.
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