ArticleThe Journal of biological chemistry2018
Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner.
Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Shared multicellular injury programs of acute and chronic kidney disease enable mechanistic patient stratification.medRxiv : the preprint server for health sciences · 2026Article
- Proteome-Wide Covalent Targeting of Acidic Residues with TunableJournal of the American Chemical Society · 2025Article
- Novel determinants of NOTCH1 trafficking and signaling in breast epithelial cells.Life science alliance · 2025Article
- An optimized fractionation method reveals insulin-induced membrane surface localization of GLUT1 to increase glycolysis in LβT2 cells.Molecular and cellular endocrinology · 2025Article
- Identification of epidermal growth factor receptor-tyrosine kinase inhibitor targeting the VP1 pocket of human rhinovirus.Antimicrobial agents and chemotherapy · 2024Article
- Influenza A virus exploits transferrin receptor recycling to enter host cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- EGFR Pathway Expression Persists in Recurrent Glioblastoma Independent of Amplification Status.Cancers · 2023Article
- RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes.The Journal of cell biology · 2023Article
- Spatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics.Nature communications · 2022Article
- RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.Nature communications · 2022Article
- DNA Methylation Levels in Mononuclear Leukocytes from the Mother and Her Child Are Associated with IgE Sensitization to Allergens in Early Life.International journal of molecular sciences · 2021Article
- The RUFYs, a Family of Effector Proteins Involved in Intracellular Trafficking and Cytoskeleton Dynamics.Frontiers in cell and developmental biology · 2020Review
- Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway.PloS one · 2020Article
- Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine.Beilstein journal of nanotechnology · 2020Review
- The endosomal sorting adaptor HD-PTP is required for ephrin-B:EphB signalling in cellular collapse and spinal motor axon guidance.Scientific reports · 2019Article
- DNA methylation changes related to nutritional deprivation: a genome-wide analysis of population and in vitro data.Clinical epigenetics · 2019Article
- The metastasis suppressor NDRG1 down-regulates the epidermal growth factor receptor via a lysosomal mechanism by up-regulating mitogen-inducible gene 6.The Journal of biological chemistry · 2019Article
- Dissecting the role of His domain protein tyrosine phosphatase/PTPN23 and ESCRTs in sorting activated epidermal growth factor receptor to the multivesicular body.Biochemical Society transactions · 2018Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that is an integral component of proliferative signaling. EGFRs on the cell surface become activated upon EGF binding and have an increased rate of endocytosis. Once in the cytoplasm, the EGF·EGFR complex is trafficked to the lysosome for degradation, and signaling is terminated. During trafficking, the EGFR kinase domain remains active, and the internalized EGFR can continue signaling to downstream effectors. Although effector activity varies based on the EGFR's endocytic location, it is not clear how this occurs. In an effort to identify proteins that uniquely associate with the internalized, liganded EGFR in the early endosome, we developed an early endosome isolation strategy to analyze their protein composition. Post-nuclear supernatant from HeLa cells stimulated with and without EGF were separated on an isotonic 17% Percoll gradient. The gradient was fractionated, and early endosomal fractions were pooled and immunoisolated with an EEA1 mAb. The isolated endosomes were validated by immunoblot using antibodies against organelle-specific marker proteins and transmission EM. These early endosomes were also subjected to LC-MS/MS for proteomic analysis. Five proteins were detected in endosomes in a ligand-dependent manner: EGFR, RUFY1, STOML2, PTPN23, and CCDC51. Knockdown of RUFY1 or PTPN23 by RNAi indicated that both proteins play a role in EGFR trafficking. These experiments indicate that endocytic trafficking of activated EGFR changes the protein composition, membrane trafficking, and signaling potential of the early endosome.
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