ArticleMethods in enzymology2018
Methods for Controlled Protein Depletion to Study Protein Function during Meiosis.
Article in Methods in enzymology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Natural diversity of telomere length distributions across 100Genome research · 2026Article
- Rapamycin-Induced Translocation of Meiotic Nuclear Proteins in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dna2 removes toxic ssDNA-RPA filaments generated from meiotic recombination-associated DNA synthesis.Nucleic acids research · 2023Article
- Article
- Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombination.Molecular cell · 2021Article
- ESA1 regulates meiotic chromosome axis and crossover frequency via acetylating histone H4.Nucleic acids research · 2021Article
- Interplay between Pds5 and Rec8 in regulating chromosome axis length and crossover frequency.Science advances · 2021Article
- C-Terminal HA Tags Compromise Function and Exacerbate Phenotypes ofG3 (Bethesda, Md.) · 2020Article
- Unresolved Recombination Intermediates Cause aGenetics · 2019Article
- S. cerevisiae Srs2 helicase ensures normal recombination intermediate metabolism during meiosis and prevents accumulation of Rad51 aggregates.Chromosoma · 2019Article
- Noncanonical Contributions of MutLγ to VDE-Initiated Crossovers DuringG3 (Bethesda, Md.) · 2019Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Proteins with potential roles in meiotic recombination often have essential or important functions during the mitotic cell cycle. In addition, proteins may have different functions at different times during meiosis. In such cases, it can be challenging to precisely determine protein function during meiosis using null or hypomorphic mutants. One example is the Sgs1-Top3-Rmi1 helicase-decatenase complex, which is required for normal vegetative growth and genome stability. In such cases, conditional loss-of-function mutants can be useful. In this chapter, we describe the construction of two types of conditional mutants, meiotic depletion alleles and auxin-induced degradation alleles, that allow protein depletion specifically during budding yeast meiosis, and illustrate their use with Sgs1. We also describe a modified method for the isolation of meiotic recombination intermediates that combines previous psoralen cross-linking and cetyltrimethylammonium bromide isolation methods.
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