ArticleMethods in enzymology2018
A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae.
Article in Methods in enzymology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- R-loops and D-loops: a delicate balance in genomic stability and instability.Cell communication and signaling : CCS · 2026Review
- Condensin loop extrusion properties, roadblocks, and role in homology search during recombination in S. cerevisiae.The EMBO journal · 2026Article
- Rad51 determines pathway usage in post-replication repair.Nature communications · 2026Article
- Donor transcription suppresses D-loops in cis and promotes genome stability.The EMBO journal · 2025Article
- Multifaceted roles of H2B mono-ubiquitylation in D-loop metabolism during homologous recombination repair.Nucleic acids research · 2025Article
- Multi-step control of homologous recombination via Mec1/ATR suppresses chromosomal rearrangements.The EMBO journal · 2024Article
- Delineation of two multi-invasion-induced rearrangement pathways that differently affect genome stability.Genes & development · 2023Article
- Delineation of two multi-invasion-induced rearrangement pathways that differently affect genome stability.bioRxiv : the preprint server for biology · 2023Article
- Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae.Journal of visualized experiments : JoVE · 2022Article
- Break-induced replication: unraveling each step.Trends in genetics : TIG · 2022Review
- Article
- Physical and Genetic Assays for the Study of DNA Joint Molecules Metabolism and Multi-invasion-Induced Rearrangements in S. cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Dynamic Processing of Displacement Loops during Recombinational DNA Repair.Molecular cell · 2019Article
- Characterizing meiotic chromosomes' structure and pairing using a designer sequence optimized for Hi-C.Molecular systems biology · 2018Article
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Authors and funding
3 authors.
Funding
Abstract
Homologous recombination faithfully restores the sequence information interrupted by a DNA double-strand break by referencing an intact DNA molecule as a template for repair DNA synthesis. DNA synthesis is primed from 3'-OH end of the invading DNA strand in the displacement loop (D-loop). Here, we describe a simple and quantitative proximity ligation-based assay to study the initiation of homologous recombination-associated DNA synthesis initiated at the D-loop and final product formation. The D-loop extension assay overcomes the semiquantitative nature and some limitations of the current PCR-based technique and facilitates the study of the recombination-associated DNA synthesis.
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