Evidence map›Paper›PMID 29522849›Full record

ArticleThe Journal of allergy and clinical immunology2018

Knob protein enhances epithelial barrier integrity and attenuates airway inflammation.

Sung Gil Ha, Mythili Dileepan, Xiao Na Ge, Bit Na Kang, Yana G Greenberg, Amrita Rao, Girija Muralidhar, Lali Medina-Kauwe, Michael A Thompson, Christina M Pabelick and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of allergy and clinical immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Sung Gil HaDepartment of Veterinary & Biomedical Sciences, University of Minnesota, St Paul, Minn.
Mythili DileepanDepartment of Veterinary & Biomedical Sciences, University of Minnesota, St Paul, Minn.
Xiao Na GeDepartment of Veterinary & Biomedical Sciences, University of Minnesota, St Paul, Minn.
Bit Na KangDepartment of Veterinary & Biomedical Sciences, University of Minnesota, St Paul, Minn.
Yana G GreenbergDepartment of Veterinary & Biomedical Sciences, University of Minnesota, St Paul, Minn.
Amrita RaoDepartment of Veterinary & Biomedical Sciences, University of Minnesota, St Paul, Minn.
Girija MuralidharLa Jolla Institute for Molecular Medicine, La Jolla, Calif.
Lali Medina-KauweDepartment of Biomedical Sciences, Cedars-Sinai Medical Center and Geffen School of Medicine, University of California-Los Angeles, Los Angeles, Calif.
Michael A ThompsonDepartment of Anesthesiology, Mayo Clinic, Rochester, Minn.
Christina M PabelickDepartments of Anesthesiology and Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minn.
Scott M O'GradyDepartments of Animal Science and Integrative Biology and Physiology, University of Minnesota, St Paul, Minn.
Savita P RaoDepartment of Veterinary & Biomedical Sciences, University of Minnesota, St Paul, Minn.
P SriramaraoDepartment of Veterinary & Biomedical Sciences, University of Minnesota, St Paul, Minn; Department of Medicine, University of Minnesota, Minneapolis, Minn. Electronic address: psrao@umn.edu.
University of Minnesota · USMayo Clinic in Arizona · USCedars-Sinai Medical Center · USInstitute for Molecular Medicine · US

Funding

UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
Tumor Targeted Corroles for Detection and InterventionR01CA140995 · NCI · CEDARS-SINAI MEDICAL CENTER · PI MEDINA-KAUWE, LALI K · 2010 to 2021
$3.7M
Protein-DNA Drug Carriers for Tumor TargetingR01CA129822 · NCI · CEDARS-SINAI MEDICAL CENTER · PI MEDINA-KAUWE, LALI K · 2009 to 2020
$3.0M
NCATS NIH HHS UL1 TR000124NCI NIH HHS R01 CA129822NCI NIH HHS R01 CA140995
6 · The paper itself

Abstract

backgroundAltered epithelial physical and functional barrier properties along with T

objectiveWe sought to determine the immunomodulatory effect of knob protein of the adenoviral capsid on allergic asthma and to investigate its mechanism of action on airway epithelial junction proteins and barrier function.

methodsAirway inflammation, including junction protein expression, was evaluated in allergen-challenged mice with and without treatment with knob. Human bronchial epithelial cells were exposed to knob, and its effects on expression of junction proteins and barrier integrity were determined.

resultsAdministration of knob to allergen-challenged mice suppressed airway inflammation (eosinophilia, airway hyperresponsiveness, and IL-5 levels) and prevented allergen-induced loss of airway epithelial occludin and E-cadherin expression. Additionally, knob decreased expression of T

conclusionIncreased expression of junction proteins mediated by knob leading to enhanced epithelial barrier function might mitigate the allergen-induced airway inflammatory response, including asthma.

Indexed as

AdenoviridaeAgedAnimalsBronchiBronchoalveolar Lavage FluidCadherinsCapsid ProteinsCell LineCytokinesEosinophiliaEpithelial CellsFemaleHumansMaleMice, Inbred BALB CMiddle AgedCadherinsCapsid ProteinsCytokinesOccludinRecombinant Proteinsadenoviral capsidairway epitheliumallergic airway inflammationasthmabarrier integrityE-cadherinKnob proteinoccludin

Identifiers

PMID29522849
PMCPMC6126992
OpenAlexW2793599405

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.