Evidence map›Paper›PMID 29520710›Full record

ArticleJournal of the American Society for Mass Spectrometry2018

A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction.

Yuejie Zhao, Jeong Yeh Yang, David F Thieker, Yongmei Xu, Chengli Zong, Geert-Jan Boons, Jian Liu, Robert J Woods, Kelley W Moremen, I Jonathan Amster

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Collision-Induced Unfolding Is Sensitive to the Polarity of Proteins and Protein Complexes.Journal of the American Society for Mass Spectrometry · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Yuejie ZhaoDepartment of Chemistry, University of Georgia, Athens, GA, 30602, USA.
Jeong Yeh YangComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
David F ThiekerComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
Yongmei XuEshelman School of Pharmacy, Division of Chemical Biology & Medicinal Chemistry, University of North Carolina, Chapel Hill, NC, USA.
Chengli ZongComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
Geert-Jan BoonsComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
Jian LiuEshelman School of Pharmacy, Division of Chemical Biology & Medicinal Chemistry, University of North Carolina, Chapel Hill, NC, USA.
Robert J WoodsComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
Kelley W MoremenComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
I Jonathan AmsterDepartment of Chemistry, University of Georgia, Athens, GA, 30602, USA. jamster@uga.edu.ORCID http://orcid.org/0000-0001-7523-5144
University of Georgia · USUniversity of North Carolina at Chapel Hill · US

Funding

TR&D3. Approaches to Proteoglycan Function at Biochemical and Cellular LevelsP41GM103390 · NIGMS · UNIVERSITY OF GEORGIA · PI MOREMEN, KELLEY W. · 2012 to 2019
$14.5M
Tools to enable non-specialists to model glycoconjugates and glycan-protein interactionsU01CA207824 · NCI · UNIVERSITY OF GEORGIA · PI FOLEY, B. LACHELE, WOODS, ROBERT J · 2016 to 2018
$1.7M
NCI NIH HHS U01 CA207824NIGMS NIH HHS P41 GM103390NIGMS NIH HHS P41GM103390NIH HHS U01 CA207824
6 · The paper itself

Abstract

Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the nonneuronal tissues. This interaction is known to be critically regulated by heparan sulfate (HS). Previous studies suggest that HS is required to promote the binding of Robo1 to Slit to form the minimal signaling complex, but the molecular details and the structural requirements of HS for this interaction are still unclear. Here, we describe the application of traveling wave ion mobility spectrometry (TWIMS) to study the conformational details of the Robo1-HS interaction. The results suggest that Robo1 exists in two conformations that differ by their compactness and capability to interact with HS. The results also suggest that the highly flexible interdomain hinge region connecting the Ig1 and Ig2 domains of Robo1 plays an important functional role in promoting the Robo1-Slit interaction. Moreover, variations in the sulfation pattern and size of HS were found to affect its binding affinity and selectivity to interact with different conformations of Robo1. Both MS measurements and CIU experiments show that the Robo1-HS interaction requires the presence of a specific size and pattern of modification of HS. Furthermore, the effect of N-glycosylation on the conformation of Robo1 and its binding modes with HS is reported. Graphical Abstract ᅟ.

Indexed as

Heparan SulfateHumansIon Mobility SpectrometryMolecular Docking SimulationNerve Tissue ProteinsProtein BindingProtein ConformationProtein DomainsProtein Interaction MapsReceptors, ImmunologicRoundabout ProteinsHeparan SulfateNerve Tissue ProteinsReceptors, ImmunologicRoundabout ProteinsSLIT1 protein, humanCarbohydratesGlycosaminoglycanIon mobilityNative sprayProtein conformationTWIMS

Identifiers

PMID29520710
PMCPMC6004239
OpenAlexW2790065372

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.