Evidence map›Paper›PMID 29520682›Full record

ArticleJournal of biomolecular NMR2018

Deuteration and selective labeling of alanine methyl groups of β

Yutaka Kofuku, Tomoki Yokomizo, Shunsuke Imai, Yutaro Shiraishi, Mei Natsume, Hiroaki Itoh, Masayuki Inoue, Kunio Nakata, Shunsuke Igarashi, Hideyuki Yamaguchi and 4 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 35 citations in OpenAlex.

  1. A random fractionalMagnetic resonance letters · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Yutaka KofukuGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Tomoki YokomizoGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Shunsuke ImaiGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Yutaro ShiraishiGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Mei NatsumeGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Hiroaki ItohGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Masayuki InoueGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Kunio NakataInstitute for Innovation, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan.
Shunsuke IgarashiInstitute for Innovation, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan.
Hideyuki YamaguchiInstitute for Innovation, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan.
Toshimi MizukoshiInstitute for Innovation, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan.
Ei-Ichiro SuzukiInstitute for Innovation, Ajinomoto Co., Inc., Kawasaki, 210-8681, Japan.
Takumi UedaGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Ichio ShimadaGraduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan. shimada@iw-nmr.f.u-tokyo.ac.jp.
The University of Tokyo · JPAjinomoto (Japan) · JPJapan Biological Informatics Consortium · JPJapan Science and Technology Agency · JP

Funding

Japan Agency for Medical Research and Development development of core technologies for innovative drug development based upon ITJapan Society for the Promotion of Science JP17H04999Japan Society for the Promotion of Science JP17H06097Japan Society for the Promotion of Science JP17J11420Ministry of Education, Culture, Sports, Science and Technology JP16H01353Ministry of Education, Culture, Sports, Science and Technology JP16H01531
6 · The paper itself

Abstract

G protein-coupled receptors (GPCRs) exist in equilibrium between multiple conformations, and their populations and exchange rates determine their functions. However, analyses of the conformational dynamics of GPCRs in lipid bilayers are still challenging, because methods for observations of NMR signals of large proteins expressed in a baculovirus-insect cell expression system (BVES) are limited. Here, we report a method to incorporate methyl-

Indexed as

AlanineAnimalsBaculoviridaeDeuteriumInsectaLipid BilayersMicellesNuclear Magnetic Resonance, BiomolecularProtein BindingProtein ConformationReceptors, Adrenergic, beta-2Staining and LabelingAlanineDeuteriumLipid BilayersMicellesReceptors, Adrenergic, beta-2AlanineBaculovirus-insect cell expression systemG protein-coupled receptorMembrane proteinMethyl TROSY

Identifiers

PMID29520682
OpenAlexW2793098662

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.