ArticleScientific reports2018
Modulating the water channel AQP4 alters miRNA expression, astrocyte connectivity and water diffusion in the rodent brain.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
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- Changes in Cx43 and AQP4 Proteins, and the Capture of 3 kDa Dextran in Subpial Astrocytes of the Rat Medial Prefrontal Cortex after Both Sham Surgery and Sciatic Nerve Injury.International journal of molecular sciences · 2024Article
- Analysis of miRNA Expression Profiles in Traumatic Brain Injury (TBI) and Their Correlation with Survival and Severity of Injury.International journal of molecular sciences · 2024Article
- Perspective and Therapeutic Potential of the Noncoding RNA-Connexin Axis.International journal of molecular sciences · 2024Review
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- Glymphatic and lymphatic communication with systemic responses during physiological and pathological conditions in the central nervous system.Communications biology · 2024Review
- AQP4 is an Emerging Regulator of Pathological Pain: A Narrative Review.Cellular and molecular neurobiology · 2023Review
- Do astrocytes act as immune cells after pediatric TBI?Neurobiology of disease · 2023Review
- Ion Channels in Gliomas-From Molecular Basis to Treatment.International journal of molecular sciences · 2023Review
- Glioblastoma disrupts the ependymal wall and extracellular matrix structures of the subventricular zone.Fluids and barriers of the CNS · 2022Article
- Microphysiological Systems for Neurodegenerative Diseases in Central Nervous System.Micromachines · 2020Review
- Regulation of AQP4 in the Central Nervous System.International journal of molecular sciences · 2020Review
- Article
- Serum Metabolomics Associating With Circulating MicroRNA Profiles Reveal the Role of miR-383-5p in Rat Hippocampus Under Simulated Microgravity.Frontiers in physiology · 2020Article
- Inhibitors of Mammalian Aquaporin Water Channels.International journal of molecular sciences · 2019Review
- The Emerging Role of microRNAs in Aquaporin Regulation.Frontiers in chemistry · 2018Review
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Aquaporins (AQPs) facilitate water diffusion through the plasma membrane. Brain aquaporin-4 (AQP4) is present in astrocytes and has critical roles in normal and disease physiology. We previously showed that a 24.9% decrease in AQP4 expression after in vivo silencing resulted in a 45.8% decrease in tissue water mobility as interpreted from magnetic resonance imaging apparent diffusion coefficients (ADC). Similar to previous in vitro studies we show decreased expression of the gap junction protein connexin 43 (Cx43) in vivo after intracortical injection of siAQP4 in the rat. Moreover, siAQP4 induced a loss of dye-coupling between astrocytes in vitro, further demonstrating its effect on gap junctions. In contrast, silencing of Cx43 did not alter the level of AQP4 or water mobility (ADC) in the brain. We hypothesized that siAQP4 has off-target effects on Cx43 expression via modification of miRNA expression. The decreased expression of Cx43 in siAQP4-treated animals was associated with up-regulation of miR224, which is known to target AQP4 and Cx43 expression. This could be one potential molecular mechanism responsible for the effect of siAQP4 on Cx43 expression, and the resultant decrease in astrocyte connectivity and dramatic effects on ADC values and water mobility.
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Registered trials
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