ArticleACS synthetic biology2018
Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779.
Article in ACS synthetic biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- CRISPR-enhanced microalgae for carbon sequestration and carotenoid production: opportunities and fundamental constraints.Bioresources and bioprocessing · 2026Review
- High-throughput Raman-activated cell sorting of microalgal genome-wide edited library revealed a regulatory pathway for carotenoid synthesis.Nature communications · 2026Article
- Review
- Enhancing vibriosis vaccination in fish: genetically modified Nannochloropsis sp. as an oral vaccine carrier.World journal of microbiology & biotechnology · 2026Article
- Synthetic Biology and Metabolic Engineering of Microalgae for Sustainable Lipid and Terpenoid Production: An Updated Perspective.Plant biotechnology journal · 2026Review
- A modular synthetic biology toolkit unlocks metabolic engineering of the industrially relevant alga Nannochloropsis.Advanced biotechnology · 2026Article
- Cross-species dissection of saline-related genes by genetically deciphering a euryhaline microalga Chlorella sp.Nature communications · 2026Article
- Harnessing microalgae for bioproducts: innovations in synthetic biology.World journal of microbiology & biotechnology · 2025Review
- Multi-dimensional epigenomic dynamics converge on H3K4-mediated regulation of low-COPlant communications · 2025Article
- Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system.Scientific reports · 2025Article
- The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges, and perspectives.Plant communications · 2025Review
- Enhanced thermotolerance via overexpression of a stromal ascorbate peroxidase in Nannochloropsis oceanica.Plant cell reports · 2025Article
- Systematic approach for dissecting promoters and designing transform systems in microalgae.Microbial cell factories · 2025Article
- Article
- Metabolic regulation strategies for enhancing microbial docosahexaenoic acid production by Schizochytrium sp.World journal of microbiology & biotechnology · 2025Review
- In Vitro Culture, Genetic Transformation and the Production of Biopharmaceuticals in Microalgae.International journal of molecular sciences · 2025Review
- Progress and challenges in CRISPR/Cas applications in microalgae.Journal of microbiology (Seoul, Korea) · 2025Review
- Article
- Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan.Nature communications · 2024Article
- Review
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Authors and funding
5 authors.
Funding
Abstract
Utilization of microalgae has been hampered by limited tools for creating loss-of-function mutants. Furthermore, modified strains for deployment into the field must be free of antibiotic resistance genes and face fewer regulatory hurdles if they are transgene free. The oleaginous microalga, Nannochloropsis oceanica CCMP1779, is an emerging model for microalgal lipid metabolism. We present a one-vector episomal CRISPR/Cas9 system for N. oceanica that enables the generation of marker-free mutant lines. The CEN/ARS6 region from Saccharomyces cerevisiae was included in the vector to facilitate its maintenance as circular extrachromosal DNA. The vector utilizes a bidirectional promoter to produce both Cas9 and a ribozyme flanked sgRNA. This system efficiently generates targeted mutations, and allows the loss of episomal DNA after the removal of selection pressure, resulting in marker-free nontransgenic engineered lines. To test this system, we disrupted the nitrate reductase gene ( NR) and subsequently removed the CRISPR episome to generate nontransgenic marker-free nitrate reductase knockout lines (NR-KO).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.