ArticleJournal of cellular and molecular medicine2018
Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells.
Article in Journal of cellular and molecular medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Intracellular chloride channel 1 and tumor.American journal of cancer research · 2023Review
- Mitochondrial gene expression signature predicts prognosis of pediatric acute myeloid leukemia patients.Frontiers in oncology · 2023Article
- Chloride Intracellular Channel 1 is a Potential Biomarker for Breast Cancer.Breast cancer (Dove Medical Press) · 2022Article
- The Role of Chloride Channels in the Multidrug Resistance.Membranes · 2021Review
- Specific binding-induced modulation of the XCL1 metamorphic equilibrium.Biopolymers · 2021Article
- Intracellular Chloride Channels: Novel Biomarkers in Diseases.Frontiers in physiology · 2020Review
- Silencing ARAF Suppresses the Malignant Phenotypes of Gallbladder Cancer Cells.BioMed research international · 2020Article
- Promising long noncoding RNA DLX6-AS1 in malignant tumors.American journal of translational research · 2020Review
- Role of ion channels in gastrointestinal cancer.World journal of gastroenterology · 2019Review
- MicroRNA‑124 negatively regulates chloride intracellular channel 1 to suppress the migration and invasion of liver cancer cells.Oncology reports · 2019Article
- Upregulated lncRNA-UCA1 contributes to metastasis of bile duct carcinoma through regulation of miR-122/Cell cycle (Georgetown, Tex.) · 2019Article
- Repurposed Biguanide Drugs in Glioblastoma Exert Antiproliferative Effects via the Inhibition of Intracellular Chloride Channel 1 Activity.Frontiers in oncology · 2019Review
- Expression of CLIC1 as a potential biomarker for oral squamous cell carcinoma: a preliminary study.OncoTargets and therapy · 2018Article
- Intracellular Chloride Ion Channel Protein-1 Expression in Clear Cell Renal Cell Carcinoma.Cancer genomics & proteomicsArticle
- CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA.Technology in cancer research & treatmentArticle
Corrections and comments
- Retraction · 2021-05-04Concerns/Issues about Data · Duplication of/in Image · Objections by Third Party · Unreliable Results and/or Conclusions ·
- RetractedRetraction.2021
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to explore the effects of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer (GBC). GBC and normal gallbladder tissues were extracted for the detection of mRNA and protein expressions of CLIC1. GBC-SD and NOZ cells in the logarithmic growth phase were selected to conduct the experiment. Three different siRNA recombined expression vectors were established using CLIC1 as a target at different sites. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting were, respectively, used to detect the CLIC1 mRNA and protein expressions. MTT assay was performed to detect the cell proliferation. Flow cytometry was applied to measure the cell apoptosis and cell cycle distribution. The variations of cell migration and invasion were evaluated using Transwell assay. GBC tissues showed higher CLIC1 mRNA and protein expressions than normal gallbladder tissues. The CLIC1 mRNA and protein expressions in the CLIC1 siRNA group were significantly lower than those in the NC and blank groups. Compared with the NC and blank groups, the CLIC1 siRNA group showed a significant decrease in cell proliferation but an obvious increase in apoptosis rate in GBC cells. Besides, in the CLIC1 siRNA group, cell percentage in G0/G1 and G2/M phase was gradually increased but decreased in S phases. The migration and invasion abilities in GBC cells were significantly lower than those in the NC and blank groups. Our study demonstrates that CLIC1 gene silencing could promote apoptosis and inhibit proliferation migration and invasion of GBC cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.