Evidence map›Paper›PMID 29516479›Full record

ArticleCurrent protocols in protein science2018

Protein Detection in Gels Using Fixation.

Lynn A Beer, David W Speicher

Abstract read
In one paragraph

Article in Current protocols in protein science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Proteome Analysis Using Gel-LC-MS/MS.Current protocols in protein science · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lynn A BeerMolecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, Pennsylvania.
David W SpeicherMolecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, Pennsylvania.

Funding

Identification of Ovarian Cancer Plasma BiomarkersR01CA131582 · NCI · WISTAR INSTITUTE · PI SPEICHER, DAVID W. · 2009 to 2018
$4.1M
Development of a serum biosignature for ectopic pregnancy.R01HD076279 · NICHD · UNIVERSITY OF PENNSYLVANIA · PI BARNHART, KURT T · 2014 to 2018
$3.4M
NCI NIH HHS R01 CA131582NICHD NIH HHS R01 HD076279
6 · The paper itself

Abstract

The most commonly used types of gels for separating proteins are SDS gels, either in a 1-D format or as the second dimension of various 2-D separations, and the most common methods of visualizing proteins in these gels use protein binding dyes after fixing the proteins in the gel matrix. In recent years, there has been a continuing trend away from preparing staining solutions in the laboratory to using commercially available kits, which are convenient, save time, have defined shelf lives, and may provide greater reproducibility than stains formulated in research laboratories. In general, when using commercial kits, satisfactory results can be readily obtained by following the manufacturer's protocols. This unit reviews commonly used fixation-based stains and provides a number of manual formulations with staining protocols for those who prefer such staining methods. © 2018 by John Wiley & Sons, Inc.

Indexed as

Coloring AgentsElectrophoresis, Gel, Two-DimensionalProteinsStaining and LabelingColoring AgentsProteinsCoomassie stainsMS compatible stainsSDS gel stainssilver stainsSYPRO Ruby

Identifiers

PMID29516479
PMCPMC6016542

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.