Evidence map›Paper›PMID 29515551›Full record

ArticleFrontiers in microbiology2018

An Experimental Insight into Extracellular Phosphatases - Differential Induction of Cell-Specific Activity in Green Algae Cultured under Various Phosphorus Conditions.

Jaroslav Vrba, Markéta Macholdová, Linda Nedbalová, Jiří Nedoma, Michal Šorf

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.9field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Jaroslav VrbaDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, České Budějovice, Czechia.
Markéta MacholdováDepartment of Ecology, Faculty of Science, Charles University, Prague, Czechia.
Linda NedbalováDepartment of Ecology, Faculty of Science, Charles University, Prague, Czechia.
Jiří NedomaInstitute of Hydrobiology, Biology Centre CAS, České Budějovice, Czechia.
Michal ŠorfDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, České Budějovice, Czechia.
Charles University · CZUniversity of South Bohemia in České Budějovice · CZInstitute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular phosphatase activity (PA) has been used as an overall indicator of P depletion in lake phytoplankton. However, detailed insights into the mechanisms of PA regulation are still limited, especially in the case of acid phosphatases. The novel substrate ELF97 phosphate allows for tagging PA on single cells in an epifluorescence microscope. This fluorescence-labeled enzyme activity (FLEA) assay enables for autecological studies in natural phytoplankton and algal cultures. We combined the FLEA assay with image analysis to measure cell-specific acid PA in two closely related species of the genus

Indexed as

acid phosphataseCoccomyxaELF97 phosphateFLEA techniqueimage cytometryinorganic phosphorusorganic phosphorusphosphorus limitation

Identifiers

PMID29515551
PMCPMC5826342
OpenAlexW2793484583

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.