ArticleBritish journal of cancer2018
Mapping of the three-dimensional lymphatic microvasculature in bladder tumours using light-sheet microscopy.
Article in British journal of cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Occlusion enhanced pan-cancer classification via deep learning.BMC bioinformatics · 2024Article
- Three-dimensional imaging of upper tract urothelial carcinoma improves diagnostic yield and accuracy.JCI insight · 2024Article
- Volumetric imaging of the tumor microvasculature reflects outcomes and genomic states of clear cell renal cell carcinoma.The journal of pathology. Clinical research · 2024Article
- Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain.eLife · 2024Article
- Article
- Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023Review
- Tissue clearing and immunostaining to visualize the spatial organization of vasculature and tumor cells in mouse liver.Frontiers in oncology · 2023Article
- Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and time.Frontiers in immunology · 2023Review
- 3D imaging for driving cancer discovery.The EMBO journal · 2022Review
- Tissue clearing to examine tumour complexity in three dimensions.Nature reviews. Cancer · 2021Review
- Tutorial: methods for three-dimensional visualization of archival tissue material.Nature protocols · 2021Review
- 3D Whole-Brain Imaging Approaches to Study Brain Tumors.Cancers · 2021Review
- Harnessing non-destructive 3D pathology.Nature biomedical engineering · 2021Review
- Multiplexed Tissue Tomography.Methods in molecular biology (Clifton, N.J.) · 2021Article
- 3D histopathology of human tumours by fast clearing and ultramicroscopy.Scientific reports · 2020Article
- Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies.Nature biomedical engineering · 2020Article
- Volumetric imaging: a potential tool to stage upper tract urothelial carcinoma.World journal of urology · 2019Article
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Authors and funding
9 authors.
Funding
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Abstract
backgroundCancers are heterogeneous and contain various types of irregular structures that can go undetected when examining them with standard two-dimensional microscopes. Studies of intricate networks of vasculature systems, e.g., the tumour lymphatic microvessels, benefit largely from three-dimensional imaging data analysis.
methodsThe new DIPCO (Diagnosing Immunolabeled Paraffin-Embedded Cleared Organs) imaging platform uses three-dimensional light-sheet microscopy and whole-mount immunolabelling of cleared samples to study proteins and micro-anatomies deep inside of tumours.
resultsHere, we uncovered the whole three-dimensional lymphatic microvasculature of formalin-fixed paraffin-embedded (FFPE) tumours from a cohort of 30 patients with bladder cancer. Our results revealed more heterogeneous spatial deviations in more advanced bladder tumours. We also showed that three-dimensional imaging could determine tumour stage and identify vascular or lymphatic system invasion with higher accuracy than standard two-dimensional histological diagnostic methods. There was no association between sample storage times and outcomes, demonstrating that the DIPCO pipeline could be successfully applied on old FFPE samples.
conclusionsStudying tumour samples with three-dimensional imaging could help us understand the pathological nature of cancers and provide essential information that might improve the accuracy of cancer staging.
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