ArticleScientific reports2018
Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- DuBois-Reymond prize lecture 2024: Multi-neuron patch-clamp to uncover the neuronal and synaptic physiology in the human neocortex.Pflugers Archiv : European journal of physiology · 2026Article
- Transport-related effects on intrinsic and synaptic properties of human cortical neurons: A comparative study.The Journal of physiology · 2026Article
- Focal cortical dysplasias: modeling pediatric drug-resistant epilepsy using human brain organoids.Frontiers in cellular neuroscience · 2026Review
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- Human brain slice cultures: translational applications and ethical considerations.Life science alliance · 2025Review
- Enhanced Distal Signaling in Human Hippocampal Neurons despite Lower Intrinsic Excitability.Research square · 2025Article
- Sulfate-based coagulants can suppress methanogenesis in treated oil sands fine tailings.Geochemical transactions · 2025Article
- Progress and potential of brain organoids in epilepsy research.Stem cell research & therapy · 2024Review
- In vitro human cell culture models in a bench-to-bedside approach to epilepsy.Epilepsia open · 2024Review
- Specificities of Living Human Microglial Cells.Advances in neurobiology · 2024Article
- Combinatorial gene therapy for epilepsy: Gene sequence positioning and AAV serotype influence expression and inhibitory effect on seizures.Gene therapy · 2023Article
- The Bovine Ex Vivo Retina: A Versatile Model for Retinal Neuroscience.Investigative ophthalmology & visual science · 2023Article
- The neurovasculature as a target in temporal lobe epilepsy.Brain pathology (Zurich, Switzerland) · 2023Review
- GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway.International journal of molecular sciences · 2022Article
- Article
- Does brain activity cause consciousness? A thought experiment.PLoS biology · 2022Article
- Human stem cell-derived GABAergic neurons functionally integrate into human neuronal networks.Scientific reports · 2021Article
- Spatiotemporal Correlation of Epileptiform Activity and Gene ExpressionFrontiers in molecular neuroscience · 2021Article
- Abolishing spontaneous epileptiform activity in human brain tissue through AMPA receptor inhibition.Annals of clinical and translational neurology · 2020Article
- Functional Electron Microscopy, "Flash and Freeze," of Identified Cortical Synapses in Acute Brain Slices.Neuron · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resected hippocampal tissue from patients with drug-resistant epilepsy presents a unique possibility to test novel treatment strategies directly in target tissue. The post-resection time for testing and analysis however is normally limited. Acute tissue slices allow for electrophysiological recordings typically up to 12 hours. To enable longer time to test novel treatment strategies such as, e.g., gene-therapy, we developed a method for keeping acute human brain slices viable over a longer period. Our protocol keeps neurons viable well up to 48 hours. Using a dual-flow chamber, which allows for microscopic visualisation of individual neurons with a submerged objective for whole-cell patch-clamp recordings, we report stable electrophysiological properties, such as action potential amplitude and threshold during this time. We also demonstrate that epileptiform activity, monitored by individual dentate granule whole-cell recordings, can be consistently induced in these slices, underlying the usefulness of this methodology for testing and/or validating novel treatment strategies for epilepsy.
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