ArticleScientific reports2018
Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Pooled it
- Noninvasive malaria detection beyond blood sampling.Sensors & diagnostics · 2026Review
- Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR.Practical laboratory medicine · 2026Review
- Hydrogel bead-based isothermal detection (BEAD-ID) for assessing the activity of DNA-modifying enzymes.iScience · 2024Article
- Non-Invasive Malaria Detection in Sub-Saharan Africa Using a DNA-Based Sensor System.Sensors (Basel, Switzerland) · 2024Article
- Urine and Saliva: Relevant Specimens for Malaria Diagnosis?Diagnostics (Basel, Switzerland) · 2022Review
- Rolling Circle Enhanced Detection of Specific Restriction Endonuclease Activities in Crude Cell Extracts.Sensors (Basel, Switzerland) · 2022Article
- Acceptance and perceived value of non-invasive malaria diagnostic tests in malaria-endemic countries.Malaria journal · 2021Article
- Method for Malaria Diagnosis Based on Extractions of Samples Using Non-Invasive Techniques: An Opportunity for the Nursing Clinical Practice.International journal of environmental research and public health · 2020Article
- Droplet microfluidics: fundamentals and its advanced applications.RSC advances · 2020Review
- Imported Malaria in Countries where Malaria Is Not Endemic: a Comparison of Semi-immune and Nonimmune Travelers.Clinical microbiology reviews · 2020Review
- Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications.Scientific reports · 2019Article
- Current Trends of Nanobiosensors for Point-of-Care Diagnostics.Journal of analytical methods in chemistry · 2019Review
Corrections and comments
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Authors and funding
18 authors at 4 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Malaria is among the major threats to global health with the main burden of disease being in rural areas of developing countries where accurate diagnosis based on non-invasive samples is in high demand. We here present a novel molecular assay for detection of malaria parasites based on technology that may be adapted for low-resource settings. Moreover, we demonstrate the exploitation of this assay for detection of malaria in saliva. The setup relies on pump-free microfluidics enabled extraction combined with a DNA sensor substrate that is converted to a single-stranded DNA circle specifically by topoisomerase I expressed by the malaria causing Plasmodium parasite. Subsequent rolling circle amplification of the generated DNA circle in the presence of biotin conjugated deoxynucleotides resulted in long tandem repeat products that was visualized colorimetrically upon binding of horse radish peroxidase (HRP) and addition of 3,3',5,5'-Tetramethylbenzidine that was converted to a blue colored product by HRP. The assay was directly quantitative, specific for Plasmodium parasites, and allowed detection of Plasmodium infection in a single drop of saliva from 35 out of 35 infected individuals tested. The results could be determined directly by the naked eye and documented by quantifying the color intensity using a standard paper scanner.
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