ArticleZoological research2018
AutoSeqMan: batch assembly of contigs for Sanger sequences.
Article in Zoological research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
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- Identification and characterization of a novel halolysin from Halostella sp. PRR32 with two C-terminal extensions.Extremophiles : life under extreme conditions · 2025Article
- Mitochondrial Genomes of Four Millipedes (Diplopoda: Spirostreptida and Spirobolida) Unveil Phylogenetic Novelty and Gene Rearrangement Patterns.Current issues in molecular biology · 2025Article
- Genetic polymorphisms in HMGCS1 gene and its association with slaughter characteristics, meat quality, and organ coefficients in Guizhou white goats.Animal bioscience · 2025Article
- Genomic signatures of sensory adaptation and evolution in pangolins.BMC genomics · 2024Article
- Identification and biochemical characterization of a novel halolysin from Halorubellus sp. PRR65 with a relatively high temperature activity.World journal of microbiology & biotechnology · 2024Article
- Genomic, Physiological, Biochemical, and Phenotypic Evidences Reveal a New Species, Halomicroarcula salaria sp. nov.Current microbiology · 2024Article
- Association between polymorphisms inFrontiers in veterinary science · 2024Article
- Expression, purification, and enzymatic characterization of an extracellular protease from Halococcus salifodinae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Comparative mitogenomes provide new insights into phylogeny and taxonomy of the subfamilyFrontiers in genetics · 2022Article
- Yet Another Quick Assembly, Analysis and Trimming Tool (YAQAAT): A Server for the Automated Assembly and Analysis of Sanger Sequencing Data.Journal of biomolecular techniques : JBT · 2021Article
- Phylogenetic analyses of distantly related clades of bent-toed geckos (genus Cyrtodactylus) reveal an unprecedented amount of cryptic diversity in northern and western Thailand.Scientific reports · 2021Article
- Identification of Yak'sAnimals : an open access journal from MDPI · 2020Article
- Complete Genome Sequence of Genotype Psittacine Beak and Feather Disease Virus, a Strain Identified from Budgerigars in China.Microbiology resource announcements · 2019Article
- Complete Genome Sequence of an Avian Polyomavirus Strain First Isolated from a Pigeon in China.Microbiology resource announcements · 2019Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the wide application of DNA sequencing technology, DNA sequences are still increasingly generated through the Sanger sequencing platform. SeqMan (in the LaserGene package) is an excellent program with an easy-to-use graphical user interface (GUI) employed to assemble Sanger sequences into contigs. However, with increasing data size, larger sample sets and more sequenced loci make contig assemble complicated due to the considerable number of manual operations required to run SeqMan. Here, we present the 'autoSeqMan' software program, which can automatedly assemble contigs using SeqMan scripting language. There are two main modules available, namely, 'Classification' and 'Assembly'. Classification first undertakes preprocessing work, whereas Assembly generates a SeqMan script to consecutively assemble contigs for the classified files. Through comparison with manual operation, we showed that autoSeqMan saved substantial time in the preprocessing and assembly of Sanger sequences. We hope this tool will be useful for those with large sample sets to analyze, but with little programming experience. It is freely available at https://github.com/ Sun-Yanbo/autoSeqMan.
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