ArticleJournal of cell science2018
Mitotic spindle assembly and γ-tubulin localisation depend on the integral nuclear membrane protein Samp1.
Article in Journal of cell science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- The TUBG meshwork is associated with centromere dynamics and micronuclear organization.Communications biology · 2026Article
- TMEM201 regulates tumor malignancy and immune microenvironment in lower-grade glioma.BMC medical genomics · 2026Article
- Sculpting nuclear envelope identity from the endoplasmic reticulum during the cell cycle.Nucleus (Austin, Tex.) · 2024Review
- Multiscale network analysis identifies potential receptors for SARS-CoV-2 and reveals their tissue-specific and age-dependent expression.FEBS letters · 2023Article
- Causal and Candidate Gene Variants in a Large Cohort of Women With Primary Ovarian Insufficiency.The Journal of clinical endocrinology and metabolism · 2022Observational
- The Diverse Cellular Functions of Inner Nuclear Membrane Proteins.Cold Spring Harbor perspectives in biology · 2021Review
- Nuclear envelope remodelling during mitosis.Current opinion in cell biology · 2021Review
- Non-Canonical Functions of the Gamma-Tubulin Meshwork in the Regulation of the Nuclear Architecture.Cancers · 2020Review
- Bioinformatics analysis of the biological changes involved in the osteogenic differentiation of human mesenchymal stem cells.Journal of cellular and molecular medicine · 2020Article
- Lamin A/C Assembly Defects inCells · 2020Article
- Choreography of the centrosome.Heliyon · 2020Review
- The Biology of the Nuclear Envelope and Its Implications in Cancer Biology.International journal of molecular sciences · 2019Review
- Review
- Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.Frontiers in synaptic neuroscience · 2019Review
- Article
- γ-Tubulin⁻γ-Tubulin Interactions as the Basis for the Formation of a Meshwork.International journal of molecular sciences · 2018Review
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated a possible role for the inner nuclear membrane protein Samp1 (also known as TMEM201) in the mitotic machinery. Live-cell imaging showed that Samp1a-YFP (Samp1a is the short isoform of Samp1) distributed as filamentous structures in the mitotic spindle, partially colocalising with β-tubulin. Samp1 depletion resulted in an increased frequency of cells with signs of chromosomal mis-segregation and prolonged metaphase, indicating problems with spindle assembly and/or chromosomal alignment. Consistent with this, mitotic spindles in Samp1-depleted cells contained significantly lower levels of β-tubulin and γ-tubulin, phenotypes that were rescued by overexpression of Samp1a-YFP. We found that Samp1 can bind directly to γ-tubulin and that Samp1 co-precipitated with γ-tubulin and the HAUS6 subunit of the Augmin complex in live cells. The levels of HAUS6, in the mitotic spindle also decreased after Samp1 depletion. We show that Samp1 is involved in the recruitment of HAUS6 and γ-tubulin to the mitotic spindle. Samp1 is the first inner nuclear membrane protein shown to have a function in mitotic spindle assembly.
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