Evidence map›Paper›PMID 29514322›Full record

ReviewNucleic acids research2018

Capturing the 'ome': the expanding molecular toolbox for RNA and DNA library construction.

Morgane Boone, Andries De Koker, Nico Callewaert

Abstract readReview
In one paragraph

Review in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Morgane BooneCenter for Medical Biotechnology, VIB, Zwijnaarde 9052, Belgium.
Andries De KokerCenter for Medical Biotechnology, VIB, Zwijnaarde 9052, Belgium.
Nico CallewaertCenter for Medical Biotechnology, VIB, Zwijnaarde 9052, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences. In the past decade especially, driven by the progress in the field of massively parallel sequencing, numerous studies have comprehensively assessed the impact of particular manipulations on library complexity and quality, and characterized the activities and specificities of several key enzymes used in library construction. Fortunately, careful protocol design and reagent choice can substantially mitigate many of these biases, and enable reliable representation of sequences in libraries. This review aims to guide the reader through the vast expanse of literature on the subject to promote informed library generation, independent of the application.

Indexed as

Gene LibraryBase SequenceCloning, MolecularDNADNA MethylationHigh-Throughput Nucleotide SequencingModels, GeneticNucleic Acid Amplification TechniquesPolymerase Chain ReactionRNADNARNA

Identifiers

PMID29514322
PMCPMC5888575

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.