ArticleJournal of experimental botany2018
Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- RUBY expression alters development and metabolism in Arabidopsis.Plant molecular biology · 2026Article
- Comparative transcriptomic analysis reveals distinct responses of beneficial bacterial endophytes to wild and cultivated rice root exudates.Plant cell reports · 2026Article
- Anatomical, Physiological, and Transcriptome Analyses Revealing Pod Shattering ofBiomolecules · 2025Article
- Light and Shadows: Insights from Large-Scale Visual Screens for Arabidopsis Leaf Morphology Mutants.International journal of molecular sciences · 2025Review
- Phenylacetic acid metabolism in land plants: novel pathways and metabolites.Journal of experimental botany · 2025Article
- Enhanced auxin signaling promotes root-hair growth at moderately low temperature in Arabidopsis thaliana.Plant communications · 2025Article
- Induction of microspore embryogenesis in bread wheat by mannitol pre-treatment is associated with the disruption of endogenous hormone balance and substantial accumulation of auxins.BMC plant biology · 2025Article
- Sulfonation of IAA in Urtica eliminates its DR5 auxin activity.Plant cell reports · 2024Article
- Advances in Plant Auxin Biology: Synthesis, Metabolism, Signaling, Interaction with Other Hormones, and Roles under Abiotic Stress.Plants (Basel, Switzerland) · 2024Review
- Karrikinolide1 (KARPlants (Basel, Switzerland) · 2024Article
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- Profiling of 1-aminocyclopropane-1-carboxylic acid and selected phytohormones in Arabidopsis using liquid chromatography-tandem mass spectrometry.Plant methods · 2024Article
- Genetic evidence for algal auxin production iniScience · 2024Article
- In situ separation and visualization of isomeric auxin derivatives in Arabidopsis by ion mobility mass spectrometry imaging.Analytical and bioanalytical chemistry · 2024Article
- Application of Long-Chained Auxin Conjugates Influenced Auxin Metabolism and Transcriptome Response inInternational journal of molecular sciences · 2023Article
- Auxin and abiotic stress responses.Journal of experimental botany · 2023Review
- Can plant hormonomics be built on simple analysis? A review.Plant methods · 2023Review
- Recent advances in auxin biosynthesis and homeostasis.3 Biotech · 2023Review
- Metabolic profiles of 2-oxindole-3-acetyl-amino acid conjugates differ in various plant species.Frontiers in plant science · 2023Article
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Authors and funding
8 authors.
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Abstract
Auxin (indole-3-acetic acid, IAA) plays fundamental roles as a signalling molecule during numerous plant growth and development processes. The formation of local auxin gradients and auxin maxima/minima, which is very important for these processes, is regulated by auxin metabolism (biosynthesis, degradation, and conjugation) as well as transport. When studying auxin metabolism pathways it is crucial to combine data obtained from genetic investigations with the identification and quantification of individual metabolites. Thus, to facilitate efforts to elucidate auxin metabolism and its roles in plants, we have developed a high-throughput method for simultaneously quantifying IAA and its key metabolites in minute samples (<10 mg FW) of Arabidopsis thaliana tissues by in-tip micro solid-phase extraction and fast LC-tandem MS. As a proof of concept, we applied the method to a collection of Arabidopsis mutant lines and identified lines with altered IAA metabolite profiles using multivariate data analysis. Finally, we explored the correlation between IAA metabolite profiles and IAA-related phenotypes. The developed rapid analysis of large numbers of samples (>100 samples d-1) is a valuable tool to screen for novel regulators of auxin metabolism and homeostasis among large collections of genotypes.
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