ArticlePLoS genetics2018
Arabidopsis ICK/KRP cyclin-dependent kinase inhibitors function to ensure the formation of one megaspore mother cell and one functional megaspore per ovule.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 62 citations in OpenAlex.
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- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
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- From the MMC Specification to Endosperm Cellularization in Arabidopsis: A Developmental-Handover Framework for Seed Initiation.Plants (Basel, Switzerland) · 2026Review
- Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis.The EMBO journal · 2026Article
- A novel and efficient method for producing high-purity single protoplast-derived isolates ofFrontiers in microbiology · 2026Article
- Stem cell regulators drive a G1 duration gradient during plant root development.Nature plants · 2025Article
- Auxin Orchestrates Germ Cell Specification inInternational journal of molecular sciences · 2025Review
- Comparative gametogenesis and genomic signatures associated with pollen sterility in the seedless mutant of grapevine.BMC plant biology · 2025Article
- Regulation of cell cycle in plant gametes: when is the right time to divide?Development (Cambridge, England) · 2025Review
- Cell death in bryophytes: emerging models to study core regulatory modules and conserved pathways.Annals of botany · 2024Review
- Germline β-1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana.Nature communications · 2024Article
- Twin Embryos inPlants (Basel, Switzerland) · 2024Article
- Embryo sac cellularization defects lead to supernumerary egg cells and twin embryos iniScience · 2024Article
- The miRNA822 loaded by ARGONAUTE9 modulates the monosporic female gametogenesis in Arabidopsis thaliana.Plant reproduction · 2024Article
- Revisiting the female germline cell development.Frontiers in plant science · 2024Review
- BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize.The Plant cell · 2023Article
- High-frequency synthetic apomixis in hybrid rice.Nature communications · 2022Article
- Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis.Nature communications · 2022Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most plants, the female germline starts with the differentiation of one megaspore mother cell (MMC) in each ovule that produces four megaspores through meiosis, one of which survives to become the functional megaspore (FM). The FM further develops into an embryo sac. Little is known regarding the control of MMC formation to one per ovule and the selective survival of the FM. The ICK/KRPs (interactor/inhibitor of cyclin-dependent kinase (CDK)/Kip-related proteins) are plant CDK inhibitors and cell cycle regulators. Here we report that in the ovules of Arabidopsis mutant with all seven ICK/KRP genes inactivated, supernumerary MMCs, FMs and embryo sacs were formed and the two embryo sacs could be fertilized to form two embryos with separate endosperm compartments. Twin seedlings were observed in about 2% seeds. Further, in the mutant ovules the number and position of surviving megaspores from one MMC were variable, indicating that the positional signal for determining the survival of megaspore was affected. Strikingly, ICK4 fusion protein with yellow fluorescence protein was strongly present in the degenerative megaspores but absent in the FM, suggesting an important role of ICKs in the degeneration of non-functional megaspores. The absence of or much weaker phenotypes in lower orders of mutants and complementation of the septuple mutant by ICK4 or ICK7 indicate that multiple ICK/KRPs function redundantly in restricting the formation of more than one MMC and in the selective survival of FM, which are critical to ensure the development of one embryo sac and one embryo per ovule.
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